Concern with increasing levels of emerging contaminants exists on a global scale. Three commonly observed emerging environmental contaminants: triclosan (2,4,4-trichloro-2'-hydroxydiphenyl ether), a synthetic, broad-spectrum antibacterial agent, and perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA), used in stain- and water-resistant treatments, have become distributed ubiquito...

Although schizophrenia has been shown to have a substantial component, there is a dearth of known risk alleles. Furthermore, all of the known risk genes are of small effect sizes. Previously it has been shown that a 12 base pair insertional polymorphism in the in the C-terminal, opposite paired (Opa) domain of the MED12 gene known as MED12 represents a small but significant risk for a positive ...

The use of tetrazolium salts for metabolic studies has been dismissed on the basis of their poor penetration into fresh tissue slices. In view of the fact that the penetration of these compounds can be visualized, it was felt that knowledge of the factors involved would be important. Factors, known to influence the penetration of oxygen, were examined with respect to the tetrazolium salts. The ...

ETHNOPHARMACOLOGICAL RELEVANCE Tuberculosis (TB) caused by Mycobacterium tuberculosis remains an ongoing threat to human health. Several medicinal plants are used traditionally to treat tuberculosis in Ghana. The current study was designed to investigate the antimycobacterial activity and cytotoxicity of crude extracts from five selected medicinal plants. MATERIAL AND METHODS The microplate a...

The interest in salt tolerance of plants, especially those of economic importance, is increasing as more saline lands are brought under cultivation, and as saline irrigation waters add to the salinity of cultivated soils. Research relating to the survival and growth of plants growing on soils containing excessive soluble salts has left many problems unsolved. The task of obtaining and selecting...

Tetrazolium salts (TS) have been previously used for radiochromic dosimetry in solutions, films and three dimensional (3D) gelatine-based gels. However, widespread application for 3D dosimetry has not been achieved due to the required high concentrations and associated high costs of the TS dimer used in prior research. Through careful selection of TS monomer, sensitivity-enhancing additives and...

The Wnt signaling pathway is activated in hepatocellular carcinoma (HCC). This study investigated the effects of FH535, an inhibitor of the Wnt signaling pathway, on the proliferation and motility of HCC cells. HLF cells and PLC/PRF/5 cells, HCC cells, were subjected to 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt (MTS) assay with the addi...

PURPOSE To improve the treatment of women with ovarian cancer, we are investigating the modulation of a prominent DNA-damaging agent, temozolomide, by manipulating the DNA base excision repair (BER) pathway via BER inhibitor, methoxyamine, and overexpression of N-methylpurine DNA glycosylase (MPG). EXPERIMENTAL DESIGN Enhancement of temozolomide via methoxyamine and MPG overexpression was ana...

Marine molluscs from the family Muricidae are the source of a homeopathic remedy Murex, which is used to treat a range of conditions, including cancer. The aim of this study was to evaluate the in vitro bioactivity of egg mass extracts of the Australian muricid Dicathais orbita, in comparison to the Murex remedy, against human carcinoma and lymphoma cells. Liquid chromatography coupled with mas...

Plant bioactives [6]-gingerol (GING), epigallocatechin gallate (EGCG) and asiaticoside (AS) and vitamin E, such as tocotrienol-rich fraction (TRF), have been reported to possess anticancer activity. In this study, we investigated the apoptotic properties of these bioactive compounds alone or in combination on glioma cancer cells. TRF, GING, EGCG and AS were tested for cytotoxicity on glioma cel...