Methyl-coenzyme M reductase (MCR) is a nickel tetrahydrocorphinoid (coenzyme F430) containing enzyme involved in the biological synthesis and anaerobic oxidation of methane. MCR catalyzes the conversion of methyl-2-mercaptoethanesulfonate (methyl-SCoM) and N-7-mercaptoheptanoylthreonine phosphate (CoB7SH) to CH4 and the mixed disulfide CoBS-SCoM. In this study, the reaction of MCR from Methanot...

the direct three component modified mannich reaction via condensation of aldehydes, 2-naphthol or 2,7-naphthalendiol and piperidine to generate betti bases has been carried out over l-proline (20 mol%) with high efficiency under solvent free conditions at 70 oc. also, the reaction of aromatic aldehyde and 4-hydroxycoumarin in the presence of l-proline (20 mol%) in the ethanol under reflux condi...

The water paradigm has changed in organic chemistry. Multiple organic reactions have been implemented in the form of watercompatible processes with a net gain in efficiency and instrumental simplicity. Most of these reactions are currently performed either as homogeneous solutions or easily-stirred aqueous suspensions. Homogeneity requires either the use of water soluble reactants or the aid of...

The rapid generation of diverse sets of complex molecules can be achieved by employing diversity oriented synthetic strategies in combination with so called complexity generating reactions. Multicomponent reactions (MCRs) have been emerged as an extremely powerful tool in combinatorial chemistry and drug discovery, since it offers significant advantages over conventional linear step syntheses, ...

The emergence of the plasmid-encoded colistin-resistance gene mcr-1 in Enterobacteriaceae represents a new threat to the treatment of infection in the clinical setting. A sensitive and rapid molecular method for detection of the mcr-1 gene in clinical isolates is needed to control the spread of this gene. In this study, we established a loop-mediated isothermal amplification (LAMP) assay for ra...

Background and aimPlasmid-mediated colistin resistance mechanisms have been identified worldwide in the past years. A multiplex polymerase chain reaction (PCR) protocol for detection of all currently known transferable colistin resistance genes (mcr-1 to mcr-5, and variants) in Enterobacteriaceae was developed for surveillance or research purposes. Methods: We designed four new primer pairs to ...

3,5-Diamino-1,2,4-triazole (DAT) became a significant energetic materials intermediate, and the study of its reaction mechanism has fundamental significance in chemistry. The aim of this study is to investigate the ability of online attenuated total reflection infrared (ATR-IR) spectroscopy combined with the novel approach of hybrid hard- and soft-modelling multivariate curve resolution-alterna...

This work describes a fast, mild, convenient and simple method for preparing of nitrogen heterocyclic derivatives by MCR reaction under solvent-free condition.