نتایج جستجو برای: multiplex reverse transcriptase pcr rt

تعداد نتایج: 295068  

Journal: :BMC Infectious Diseases 2006
Marina Hoehne Eckart Schreier

BACKGROUND Due to an increasing number of norovirus infections in the last years rapid, specific, and sensitive diagnostic tools are needed. Reverse transcriptase-polymerase chain reactions (RT-PCR) have become the methods of choice. To minimize the working time and the risk of carryover contamination during the multi-step procedure of PCR the multiplex real-time RT-PCR for the simultaneous det...

Journal: :Journal of virology 2012
Werner J D Ouwendijk Alexander Choe Maria A Nagel Don Gilden Albert D M E Osterhaus Randall J Cohrs Georges M G M Verjans

We analyzed the varicella-zoster virus (VZV) transcriptome in 43 latently infected human trigeminal ganglia (TG) with postmortem intervals (PMIs) ranging from 3.7 to 24 h. Multiplex reverse transcriptase PCR (RT-PCR) revealed no VZV transcripts with a PMI of <9 h. Real-time PCR indicated a significant increase (P = 0.02) in VZV ORF63 transcript levels but not the virus DNA burden with longer PM...

Journal: :modares journal of medical sciences: pathobiology 2007
behjat gholami majid sadeghizadeh hossein najmabadi

objective: chronic myeloid leukemia (cml) is a malignant clonal disorder of hematopoietic stem cells which results in increase of myeloid cells, erythroid cells and platelets in the peripheral blood and hyperplasia in bone marrow. investigations have shown different types of bcr- abl variants in these patients. in iran only 3 types of these variants have been identified because most of the clin...

Journal: :Singapore medical journal 2007
Y K Yong R Thayan H T Chong C T Tan S D Sekaran

INTRODUCTION Dengue fever and dengue haemorrhagic fever currently rank highly among the newly-emerging infectious diseases, and are considered to be the most important arboviral disease worldwide. The definitive diagnosis is culture analysis, but practical considerations limit its use. Also, the period for viral detection is limited. Within a day or two after fever subsides, rising levels of an...

2013
Jesse J. Waggoner Janaki Abeynayake Malaya K. Sahoo Lionel Gresh Yolanda Tellez Karla Gonzalez Gabriela Ballesteros Anna M. Pierro Paolo Gaibani Frances P. Guo Vittorio Sambri Angel Balmaseda Kumudu Karunaratne Eva Harris Benjamin A. Pinsky

BACKGROUND Dengue fever results from infection with one or more of four different serotypes of dengue virus (DENV). Despite the widespread nature of this infection, available molecular diagnostics have significant limitations. The aim of this study was to develop a multiplex, real-time, reverse transcriptase-PCR (rRT-PCR) for the detection, quantitation, and serotyping of dengue viruses in a si...

Journal: :Infectious diseases and clinical microbiology 2023

Objective: Reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) is one of the time-saving, accurate, and cost-effective alternative methods to real-time polymerase chain reaction (RT-PCR). This study aimed identify robustness a colorimetric RT-LAMP assay kit that we developed, detecting SARS-COV-2 viral RNA within 30 minutes using primer set special N gene against RT-PCR, gold...

2010
Sandra J. Laney Reda M. R. Ramzy Hanan H. Helmy Hoda A. Farid Ameen A. Ashour Gary J. Weil Steven A. Williams

BACKGROUND Detection of filarial DNA in mosquitoes by PCR cannot differentiate infective mosquitoes from infected mosquitoes. In order to evaluate transmission risk an assay is needed that can specifically detect infective L3 stage parasites. We now report the development of an assay that specifically detects the infective stage of Wuchereria bancrofti in mosquitoes. The assay detects an L3-act...

2014
Mohamed S. Al-Ayed Ahmed M. Asaad Mohamed A. Qureshi Mohammed S. Ameen

OBJECTIVES To investigate 15 respiratory viruses in children with acute respiratory tract infections (ARTIs) using multiplex reverse-transcriptase polymerase chain reaction (RT-PCR), and to analyze the clinical and epidemiological features of these viruses. METHODS In a cross-sectional study, 135 children, ≤5 years of age who presented with ARTIs in Najran Maternity and Children Hospital, Naj...

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