A multiplex Taqman-based real-time reverse transcription (RT) polymerase chain reaction (PCR) assay was developed to identify potential severe strains of Citrus tristeza virus (CTV) and separate genotypes that react with the monoclonal antibody MCA13. Three strain-specific probes were developed using intergene sequences between the major and minor coat protein genes (CPi) in a multiplex reactio...

In this study, pathogenic Aeromonas hydrophila, A. veronii, and A. schubertii from fish were detected by multiplex TaqMan real-time PCR assay. The assay utilized three pairs of specific primers and three corresponding TaqMan probes designed to detect the aerolysin gene in A. hydrophila, the aerolysin gene in A. veronii, and the gyrB gene in A. schubertii. The specificity of the probe and primer...

Large-scale (temporal and/or spatial) molecular investigations of the diversity and distribution of arbuscular mycorrhizal fungi (AMF) require considerable sampling efforts and high-throughput analysis. To facilitate such efforts, we have developed a TaqMan real-time PCR assay to detect and identify AMF in environmental samples. First, we screened the diversity in clone libraries, generated by ...

Background: Crimean-Congo hemorrhagic fever virus (CCHFV) is a highly lethal that causes in humans and endemic many countries, including Iran. Therefore, fast, accurate, reliable diagnosis crucial for patient management outbreak control. Objectives: This study aims to optimize TaqMan multiplex real-time RT-PCR the rapid specific of CCHFV. Methods: In this study, L (NC_005301.3) S (NC_005302.1) ...

AIMS The aim of this study was to compare the performance of four TaqMan RT-PCR assays with a commonly used nested RT-PCR and to include the Feline calicivirus (FCV) as an internal control. METHODS AND RESULTS RNA extracted from 87 swine faecal samples and 103 swine blood samples was subjected to different detection systems. Faecal samples naturally contaminated with Hepatitis E virus (HEV) a...