Resolution of binary mixtures of theophylline (THEO), montelukast (MKST) and loratadine (LORA) with minimum sample pretreatment and without analyte separation has been successfully achieved by multivariate spectrophotometric calibration, together with partial least-squares (PLS-1), principal component regression (PCR) and hybrid linear analysis (HLA). Data of analysis were obtained from UV–Vis ...

An analytical methodology based on spectrophotometric and partial least squares (PLS) algorithm for thesimultaneous determination of ampicillin and penicillin in human plasma was developed and validated. Themultivariate model was developed as a binary calibration model and it was built and validated with anindependent set of synthesis and real samples in presence of matrix. It is shown how a de...

This paper gives an introduction to multivariate calibration from a chemometrics perspective and reviews the various proposals to generalize the well-established univariate methodology to the multivariate domain. Univariate calibration leads to relatively simple models with a sound statistical underpinning. The associated uncertainty estimation and figures of merit are thoroughly covered in sev...

attempts to obtain experimental values for the kinetic parameters of phenanthridine oxidation by guinea pig or rabbit liver aldehyde oxidase using common spectrophotometric methods have not been successful due to a lower limit of detection. in the present study, a new spectrofluorimetric assay in combination with a multivariate calibration method for enzymatic kinetic study of aldehyde oxidase ...

Problems concerning covariance among independent variables are well understood and dealt with by inverse regression methods like partial least squares regression. However, between dependent has only received minor attention. Biological samples often complex mixtures of multiple covarying compounds. During multivariate calibration, analyte predictions may be mediated through relationships interf...

Attempts to obtain experimental values for the kinetic parameters of phenanthridine oxidation by guinea pig or rabbit liver aldehyde oxidase using common spectrophotometric methods have not been successful due to a lower limit of detection. In the present study, a new spectrofluorimetric assay in combination with a multivariate calibration method for enzymatic kinetic study of aldehyde oxidase ...