A new, simple immunochromatographic assay for rapid identification of Mycobacterium tuberculosis complex in liquid cultures has been developed. The principle of the assay is binding of the Mycobacterium tuberculosis complex specific antigen to the monoclonal antibody conjugated on the test strip. The aim of this study is evaluation of the performance of immunochromatographic assay in identifica...

tuberculosis (tb) is an important health problem in developing countries, with varying clinical presentations depending on the organs/systems involved. to study the spectrum of clinical and paraclinical aspects of extra pulmonary tb in children suffering from pulmonary tb. this study has been carried out on 65 children with tuberculosis, admitted in tb wards of national research institute of tu...

In most countries, tuberculosis caused by Mycobacterium bovis is mainly a disease of cattle but can infect buffalos too. The disease can be controlled successfully by mean of a test-and-slaughter program. In Iran test-and-slaughter program has started since 1971 and prevalence of bovine tuberculosis reduces from 5% to less than 0.12% in recent years. In Western Azarbaijan, North West of Iran, t...

To better understand the biology and the virulence determinants of the two major mycobacterial human pathogens Mycobacterium tuberculosis and Mycobacterium leprae, their genome sequences have been determined recently. In silico comparisons revealed that among the 1439 genes common to both M. tuberculosis and M. leprae, 219 genes code for proteins that show no similarity with proteins from other...

SETTING Mycobacteria growth in media with the addition of inhibitory substances has been used in species identification. Growth of the Mycobacterium tuberculosis complex (MTC) is inhibited by rho-nitrobenzoic acid (PNB), whereas non-tuberculous mycobacteria (NTM) are resistant. OBJECTIVE To develop a rapid PNB test using the automated BACTEC MGIT960 system and to evaluate its usefulness in th...

OBJECTIVES To evaluate BD MGITTM TBc® technique for identifying the Mycobacterium tuberculosis complex using isolates obtained in liquid and solid media. METHODS A descriptive study was conducted in which 117 isolates were analyzed by the immune-chromatography technique obtained from solid and liquid cultures to identify the Mycobacterium tuberculosis complex. The kappa coefficient was calcul...

Mycobacterium mungi, a Mycobacterium tuberculosis complex pathogen, has emerged in banded mongoose in northern Botswana and Northwest Zimbabwe. The pathogen is transmitted through infected secretions used in olfactory communication behavior (K. A. Alexander, C. E. Sanderson, M. H. Larsen, S. Robbe-Austerman, M. C. Williams, and M. V. Palmer, mBio 7(3):e00281-16, 2016, http://dx.doi.org/10.1128/...

Spoligotyping was performed to study the population structure of Mycobacterium tuberculosis complex strains (n = 224) from Bangladesh. Strains were split into principal genetic group 1 (PGG 1 [75.0%]) and PGG 2 and 3 (25%). Forty-nine strains with a new spoligotype signature and considered as south or southeast Asian-linked emerging clones were designated as "Matlab type."