Mycoplasmas comprise a conglomerate of pathogens and commensals occurring in humans and animals. The genus Mycoplasma alone contains more than 120 species at present, and new members are continuously being discovered. Therefore, it seems promising to use a single highly parallel detection assay rather than develop separate tests for each individual species. In this study, we have designed a DNA...

Mycoplasma 352 Molecular characterization of mycoplasma gallisepticum isolated from chicken and turkey. 353 Pathogenicity of Mycoplasma gallisepticum strains using ELD50 in embryonated chicken eggs. 354 Evaluation of three DNA extraction methods for the detection of Mycoplasma spp. with an MG/MS multiplex real-time PCR method. 356 Use of MG/MS serology and PCR to determine flock status. 357 Exp...

Objective: We examined the blood of 48 North American Chronic Fatigue Syndrome (CFS) patients subsequently diagnosed with Lyme Disease Borrelia burgdorferi and compared these to 50 North American CFS patients without evidence of Borrelia burgdorferi infections for presence of Mycoplasma spp. co-infections using forensic polymerase chain reaction. Results: We found that 68.75% of CFS/Lyme patien...

Introduction Mycoplasmas are normal inhabitants of the upper respiratory airways, urinary and genital tracts, and the digestive system; however, they can become infectious and cause high impact diseases in dairy herds including mastitis and metritis. In dairy cattle, Mycoplasma bovis is the most commonly isolated mycoplasma species (spp.). This species is very invasive and capable of colonizing...

The deregulation of the 2.5A synthetase RNase L antiviral pathway and the prevalence of Mycoplasma spp. in subsets of Chronic Fatigue Syndrome [CFS] have been separately reported in the scientific literature. We hypothesised that a co-morbid pathophysiological mechanism involving infection by Mycoplasma spp. and the deregulation of the 2,5A synthetase / RNase L antiviral pathway may exist in CF...

Quinupristin/dalfopristin displays in-vitro bacteriostatic activity against all Legionella spp. (MICs = 0.06-2 mg/L), with Legionella pneumophila usually being at least two-fold more sensitive to quinupristin/dalfopristin than Legionella bozemanii, Legionella dumoffii, Legionella gormanii and Legionella micdadei (MIC = 0.06-2 vs 1-2 mg/L, respectively). Against Legionella spp., quinupristin/dal...

conclusions in conclusion, the data showed that high rate of females harbor these microorganisms in the genital microbiota. this finding suggests the importance of routine gynecological tests, mainly in pregnant females, during the second and third trimesters, and in asymptomatic females under infertility clinical investigation. furthermore, the application of molecular methods is suggested; si...

Mycoplasma species, which can cause upper respiratory tract disease (URTD), are significant pathogens of birds, mammals, fish, and reptiles. Mycoplasmosis is of high concern for chelonian conservation, with the most well-documented cases in gopher and desert tortoises. Mycoplasma sp. infections have been reported in captive and free-living box turtles ( Terrapene spp.). We documented URTD assoc...

Mycoplasma spp. are rarely recognized agents of infective endocarditis. We report a case of Mycoplasma hominis prosthetic valve endocarditis diagnosed by 16S ribosomal DNA (rDNA) PCR and culture of valves in a 74-year-old man. We reviewed the literature and found only 8 other cases reported.

We compared the Mycoplasma Duo kit (Sanofi Diagnostics Pasteur) with PCR for detection of Ureaplasma spp. in endotracheal aspirates from 60 premature neonates. The overall agreement between the two tests was 96%. The Mycoplasma Duo assay is a useful alternative to culture and PCR for detection of neonatal Ureaplasma infection.