Introduction: Multiple sclerosis (MS) is a disease of the immune system: it attacks the myelin around the axons and leaves them exposed. Destruction of myelin weakens the electrical conduction of ions and thus leads to a lack of communication in the nervous system.  Methods: In the present study, we constructed recombinant plasmid and then transformed to E. coli cell. The colonies containing...

The localization and activity of the enzyme UDP-galactose-hydroxy fatty acid-containing ceramide galactosyltransferase is described in rat brain myelin subfractions during development. Other lipid-synthesizing enzymes, such as cerebroside sulphotransferase, UDP-glucose-ceramide glucosyltransferase and CDP-choline-1,2-diacylglycerol cholinephosphotransferase, were also studied for comparison in ...

During neonatal development glucocorticoids potentiate oligodendrocyte differentiation and myelinogenesis by regulating the expression of myelin basic protein, proteolipid protein, and glycerol phosphate dehydrogenase (sn-glycerol-3-phosphate: NAD+ 2-oxidoreductase, EC 1.1.1.8). The actual locus at which hydrocortisone exerts its developmental influence on glial physiology is, however, not well...

T he major inflammatory disease of the central nervous system is multiple sclerosis (MS). It is often considered a 2-stage disease with an initial inflammatory attack on myelin involving the 2 hallmarks of adaptive immunity: antigen-specific T cells and antibodies directed to protein and lipid components of the myelin sheath (1). This concerted attack involving the adaptive immune system is fol...

Background: Progesterone as a sex steroid hormone is thought to affect and prevent demyelination, but its role in promoting myelin repair is far less investigated. In this study, remyelinating potential of progesterone in corpus callosum was evaluated on an experimental model of MS.Methods: In this experimental study, adult male C57BL/6 mice were fed with 0.2% (w/w) cuprizone in ground breeder ...

Myelin membranes prepared from rat brain possess both the enzyme and substrates to incorporate azP from (ya2P]ATP into membrane protein constituents. Qf the myelin polypeptides, only the two basic proteins were phosphorylated ; and both components appeared to be equally good substrates for endogenous or added protein kinases. The a2P was transferred primarily to serine residues of the basic pro...

Objective: Multiple sclerosis (MS) is presented with motor and sensory function loss. It is caused by demyelination and following axonal lesion. As myelin basic protein (MBP) is one of the key elements of the myelin cover, we examined the level of MBP in serum, stimulated, and unstimulated saliva as a suitable biomarker for detecting MS. Methods: A case-control study was performed in 29 health...

Gelsolin is a Ca(2+)-sensitive actin filament-severing protein. To elucidate the role of gelsolin in nervous tissues, we have investigated localization and expression of gelsolin in rat CNS and PNS using biochemical and morphological methods with a polyclonal antibody against the COOH-terminal fragment of plasma gelsolin. Immunohistochemical study showed that gelsolin was specifically enriched ...