Genetic control of embryogenesis switches from the maternal to the zygotic genome during the maternal-to-zygotic transition (MZT), when maternal mRNAs are destroyed, high-level zygotic transcription is initiated, the replication checkpoint is activated and the cell cycle slows. The midblastula transition (MBT) is the first morphological event that requires zygotic gene expression. The Drosophil...

Gene regulatory networks (GRNs) evolve as a result of the coevolutionary processes acting on transcription factors (TFs) and the cis-regulatory modules they bind. The zinc-finger TF zelda (zld) is essential for the maternal-to-zygotic transition (MZT) in Drosophila melanogaster, where it directly binds over thousand cis-regulatory modules to regulate chromatin accessibility. D. melanogaster dis...

CONTEXT Diabetic nephropathy (DN) is the leading cause of end-stage renal disease (ESRD) in the United States. OBJECTIVE The aim of this study was to determine whether there were skin fibroblast gene expression differences between subjects with type 1 diabetes (T1D) with or without DN. SETTING This was a cross-sectional study conducted in the University of Minnesota. PATIENTS Study volunt...

ABSTRACT Zygotic genomic activation (ZGA) is a landmark event in the maternal-to-zygotic transition (MZT), and regulation of ZGA by maternal factors remains to be elucidated. In this study, depletion ring finger protein 114 (RNF114), ubiquitin E3 ligase, led developmental arrest two-cell mouse embryos. Using immunofluorescence transcriptome analysis, RNF114 was proven play crucial role major ZG...

The maternal-to-zygotic transition (MZT) marks the period when the embryonic genome is activated and acquires control of development. Maternally inherited factors play a key role in this critical developmental process, which occurs at the 2-cell stage in mice. We investigated the function of the maternally inherited factor Stella (encoded by Dppa3) using single-cell/embryo approaches. We show t...

Roundness is one of the most common features in machining. The minimum zone tolerance (MZT) approach provides the minimum roundness error, i.e. the minimum distance between the two concentric reference circles containing the acquired profile; more accurate form error estimation results in less false part rejections. MZT is still an open problem and is approached here by a Genetic Algorithm. Onl...

The objective of this study was to determine the dose-dependent effects of isoflurane on various cardiovascular parameters and the stable range of isoflurane concentrations in Asiatic black bears (Ursus thibetanus). Seven Asiatic black bears were intramuscularly injected with medetomidine, zolazepam and tiletamine (MZT) to induce anesthesia, and anesthesia was maintained by administering isoflu...

In honeybees, the haplodiploid sex determination system promotes a unique embryogenesis process wherein females develop from fertilized eggs and males develop from unfertilized eggs. However, the developmental strategies of honeybees during early embryogenesis are virtually unknown. Similar to most animals, the honeybee oocytes are supplied with proteins and regulatory elements that support ear...

The marine macroalgae species are recognized as the food of future with excellent bioactive properties. This study aimed to investigate phenolic compounds; cytotoxic, antibacterial, and antioxidant activities; total (TPC) flavonoid (TFC) contents methanol, ethanol, water extracts Padina pavonica (PP) Zanardinia typus (ZT). To best our knowledge, this is first report in which ZT was evaluated te...

Mammalian oocyte maturation depends on the translational activation of stored maternal mRNAs upon meiotic resumption. Cytoplasmic polyadenylation element binding protein 1 (CPEB1) is a key oocyte factor that regulates maternal mRNA translation. However, the signal that triggers CPEB1 activation at the onset of mammalian oocyte maturation is not known. We provide evidence that a mitogen-activate...