We previously reported the existence of two different kinds of fimbriae expressed by Porphyromonas gingivalis ATCC 33277. In this study, we isolated and characterized a secondary fimbrial protein from strain FPG41, a fimA-inactivated mutant of P. gingivalis 381. FPG41 was constructed by a homologous recombination technique using a mobilizable suicide vector, and failed to express the long fimbr...

A Gram-positive, facultative anaerobic, rod-shaped, and endospore-forming strain, designated 53-2(T) was isolated from the root nodule of Oxytropis ochrocephala Bunge growing on Qilian mountain, China. The strain can grow at pH 7.0-8.0, 10-50 °C and tolerate up to 11% NaCl. Optimal growth occurred at pH 7.2 and 37 °C. The result of BLASTn search based on 16S rRNA gene sequence revealed that str...

Human epidermal growth factor (hEGF), a 6.2 kDa protein of 53 amino acids with three internal disulfide bridges, has been crystallized by the hanging-drop method. hEGF crystallizes in space group P3(1)21 (or P3(2)21) using MgCl(2) as precipitant, with unit-cell parameters a = b = 61.4, c = 87.0 A. Another type of crystal, obtained using NaCl as precipitant, belongs to a tetragonal point group a...

The oxygen evolving complex (OEC) of photosystem II (PSII) requires chloride as a critical cofactor. The Cl content of PSII was determined to be 1 Cl/PSII by the AgCl colloid assay. The dissociation constant for Cl in NaCl-washed PSII was determined using the Cl sensitive microelectrode. Two classes of Cl binding sites were found with Kd values of 53 μM and 4.2 mM. The effects of anions on the ...

Single-stranded adenovirus-associated virus type 2 deoxyribonucleic acid (AAV-2 DNA) has been isolated from the virion after enzymatic pretreatment of the particles by heating at 53 C for 1 hr in 0.015 m NaCl plus 0.0015 m sodium citrate in the presence of 1% sodium dodecyl sulfate. Double-stranded AAV-2 DNA present as a marker is not denatured by this treatment. AAV-2 single-stranded DNA is co...

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The accelerating trend toward Faster, Better, Cheaper (FEE) missions places increasing emphas is on the trade-offs between requirements and risk to reduce cost and development times, while still improving quality and reliability. The Risk/Requirenlents Trade-off Guidelines discussed in this paper are part of an integrated approach to address the main issues by focusing on the sum of prevention,...

An experiment was conducted to study the performance of rice genotypes for salt tolerance under in vitro condition. Seven genotypes include salt tolerant (Pokkali, CSR 10, TRY 1 and TRY2), moderately tolerant to salt (White Ponni and BPT 5204) and susceptible (IR 29) were used for this study through embryo culture technique. Callus was initiated in MS medium + 2 mgl 2,4D + 0.5 mgl Kin and diffe...