Abstract Poly-γ-glutamic acid (γ-PGA) and nattokinase (NK) are the main substances produced by Bacillus subtilis natto in solid-state fermentation have wide application prospects. We found that our strains had higher activity of when soybeans were used as substrate to increase yield γ-PGA. Commercial production γ-PGA requires an understanding mechanism co-production. Here, we obtained maximum (...

Nattokinase is a potent fibrinolytic enzyme, obtained from ‘natto’ a traditional food in Japan. It breaks fibrin strands both by hydrolyzing fibrin in blood clots directly and hastens the production of tissue plasminogen Activator. It was also found to be lower the blood pressure and has been used in the management of atherosclerosis, coronary artery disease, and stroke. Endothelin-1 (ET-1) is ...

Oral anticoagulation with a vitamin K antagonist like warfarin is the standard of care for thromboprophylaxis after cardiac valve replacement. However requires strict monitoring INR to avoid bleeding.Bleeding major concern warfarin, particularly in those at high risk. Nattokinase an enzyme obtained from natto, cheese-like food made soybeans fermented Bacillus subtilis, which has been consumed a...

Bacillus subtilis is a generally recognized as safe probiotic, which used starter for natto fermentation. Natto functional food with antithrombus function due to nattokinase. Compared natto, fermented milk more popular food, commonly by Lactobacillus bulgaricus and Streptococcus. However, there no report on B. subtilis–fermented milk. In this study, produce function, strain (B. JNFE0126) that p...

BACKGROUND Signal peptide peptidases play an important role in the removal of remnant signal peptides in the cell membrane, a critical step for extracellular protein production. Although these proteins are likely a central component for extracellular protein production, there has been a lack of research on whether protein secretion could be enhanced via overexpression of signal peptide peptidas...

materials and methods in the present study, a potent nk-producing strain was isolated from cow milk and identified. to enhance the yield of nk, effect of various parameters such as ph, temperature, carbon source, nitrogen source and inoculum size were optimized. strain improvement of p. aeruginosa cmss was done by random uv-mutagenesis. nattokinase was partially purified and the activity was de...

The study is of determination of various conditions for spray drying in producing synbiotic in the form of capsule from Bacillus subtilis natto. The experiments were conducted to examine effects of various factors such as the resistant starch-to-matodextrin ratio before drying, the inlet gas temperature and the inlet flow. The optimization experiments are administered: a ratio of wall materials...