Neurogenesis, recovery from nerve injury, neurodegeneration, and Parkinson's disease affect people's health, yet the underlying molecular mechanisms remain elusive. Here, we investigated the effect of ganglioside GM1 and neural growth factor (NGF) on neural stem cell (NSC) proliferation and differentiation in vitro to provide a scientific basis for comprehensive treatment of nervous system dise...

BACKGROUND The effects of lysophosphatidylcholine (LPC) on electrophysiological activities and intracellular Ca2+ concentration ([Ca2+]i) were investigated in coronary arterial smooth muscle cells (CASMCs). METHODS AND RESULTS The patch clamp techniques and Ca2+ measurements were applied to cultured rabbit CASMCs. The membrane potential was -46.0+/-5.0 mV, and LPC depolarized it. Replacement ...

The development of N-methyl-D-aspartate (NMDA)-induced burst discharge in rat trigeminal motoneurons (TMNs) between postnatal days P1 and P10 was examined using whole cell patch-clamp recording methods in brain slices. Bath application of NMDA (50 microM) induced a Mg(2+)-dependent rhythmical bursting activity starting around P8. Prior to the onset of bursting, the membrane potential depolarize...

Neural Stem Cells (NSCs) have a remarkable capacity to proliferate and differentiate to other cell types. This ability to differentiate to desirable phenotypes has motivated clinical interests, hence the interest here to segment Neural Stem Cell (NSC) clusters to locate the NSC clusters over time in a sequence of frames, and in turn to perform NSC cluster motion analysis. However the manual seg...

To study the impact of COVID-19 lockdown on cognitions and emotions felt during sexual intercourse by analyzing responses 1079 French-speaking subjects (338 men, 741 women, median age: 31 years) who participated in an online survey between April 27 May 11, 2020. Negative (NSC), positive (PSE) negative (NSE) are assessed using a tool inspired Sexual Mode Questionnaire. Data crossed with sociodem...

OBJECTIVE The mechanosensitive nonselective cation channel (NSC(MS)) and endothelin-1 (ET-1) play critical roles in the regulation of vascular tone. This study was undertaken to investigate the effect of ET-1 on NSC(MS) and on the myogenic response of arteries. METHODS Cell-attached patch-clamp techniques were applied to rabbit pulmonary and cerebral arterial smooth muscle cells using a 140 m...

We have designed a novel transcriptome subtraction method for the genome-scale analysis of differential gene expression in highly complex eukaryotes, in which suppression subtractive hybridization (SSH) is performed first to enrich the target and, after exchange of adapters, negative subtraction chain (NSC) is then used to eliminate the remaining background. NSC evolved from differential subtra...

The ion channel conductances that regulate the membrane potential was investigated by using a perforated patch-clamp technique in rabbit aorta endothelial cells (RAECs). The whole-cell current/voltage (I-V) relation showed a slight outward rectification under physiological ionic conditions. The resting membrane potential was -23.3 +/- 1.1 mV (mean +/- SEM, n = 19). The slope conductances at the...