Identical beta-lactamase-encoding (TEM-1) plasmids were found in two different clinical Neisseria meningitidis strains. They were completely sequenced (5,597 bp) and designated pAB6. The plasmid is almost identical to Neisseria gonorrhoeae plasmid pJD5 (5,599 kb) and may have been picked up from a gonococcus in vivo.

Ninety-eight per cent of laboratory isolates of Neisseria gonorrhoeae from a veneral disease clinic gave positive reactions with a gonococcal coagglutination test. The prototype reagent, however, was poor at distinguishing between different species of the genus Neisseria: 75% of strains of Neisseria meningitidis and 40% of other Neisseria species tested gave positive reactions. None of the orig...

background and objectives: neisseria meningitidis is transmitted from person-to-person. thus, close contact with a healthy carrier can facilitate the spread of the bacteria and lead to life-threatening meningococcal disease. the aim of this study was to identify oropharyngeal carriers of n. meningitidis in volunteers preparing for military service before vaccination. materials and methods: in a...

A radiometric procedure was compared with the Minitek and Cystine Trypticase Agar sugar degradation methods for identification of 113 Neisseria species (58 Neisseria meningitidis, 51 Neisseria gonorrhoeae, 2 Neisseria lactamica, 2 Neisseria sicca). Identification of meningococci and gonococci was confirmed by agglutination and fluorescent antibody techniques, respectively. The Minitek method id...

background: neisseria meninigitidis is one of the most frequently encountered microorganisms associated with central nervous system infections. the aim of this study was to evaluate a pcr-based assay for specific and rapid detection of n. meninigitidis in csf specimens.   methods : since april 2002 to july 2006, 130 csf specimens were collected from patients suspected of having bacterial mening...

A full-length heme oxygenase gene from the gram-negative pathogen Neisseria meningitidis was cloned and expressed in Escherichia coli. Expression of the enzyme yielded soluble catalytically active protein and caused accumulation of biliverdin within the E. coli cells. The purified HemO forms a 1:1 complex with heme and has a heme protein spectrum similar to that previously reported for the puri...

conclusions nucleic acid amplification tests (naats) are very appropriate in detection of infected individuals. detection techniques such as naats are independent of bacterial viability, and have a potential to limit false negative samples, therefore, in our country, the application of different laboratory diagnosis methods including naats with culture as gold standard for determination antimic...

Computational analysis, design and expression of hybrid antibody in Single Chain Fragment Variable (scFv) form for identifying surface antigen factor H binding protein (fHbp) Neisseria meningitidis

objective: sexually transmitted infections (stis) remain a public health problem of major significance in most parts of the world. this study aimed to detect the most prevalent pathogens in patients with signs and symptoms of sti referring to a group of university clinics in tehran. materials and methods: in this cross-sectional study using randomized cluster sampling, 507 consecutive male and ...

Wheat-germ agglutination (WGA) was used to identify 168 strains of Neisseria gonorrhoeae and 105 strains of other Neisseria species in a routine laboratory. About one-third of the meningococci reacted with the lectin and titres with some organisms varied on repeat testing. The technique is regarded as unreliable for the identification of Neisseria species.