Over one-year period, 150 pasture soil samples and 138 sheep faecal samples, collected from different parts of Iran were screened for the presence of nematophagous fungi. The samples were cultured at 25ºC on chloramphenicol-2% water agar (CHF-WA) plates in the presence of Haemonchus contortus third stage larvae (L3) and checked over a two-month period for characteristic conidia, conidiophores a...

Gliocladium virens was isolated from slash pine trees symptomatic and asymptomatic for pine wilt disease with frequencies of 24% and 10%, respectively. Populations of Bursaphelenchus xylophilus, the nematode incitant of this disease, reproduced on this fungus and inhibited its growth. Growth inhibition of the fungus was characterized by an absence of sporulation and by the formation of chains o...

A strain of the fungus Fusarium equiseti isolated from soybean cyst nematode secretes nematode-antagonistic compounds. Bioassay-guided fractionation of an extract of the culture broth was undertaken to identify the compounds. Fractions were assayed for activity against a root-knot nematode (Meloidogyne incognita), a plant pathogen that attacks the roots of numerous plant species. Two trichothec...

root-knot nematodes (meloidogyne spp.) are among the most important groups of plant-parasitic nematodes, causing damage and yield losses to most cultivated plants. the bacterium pseudomonas fluorescens cha0 and the fungus trichoderma harzianum bi were investigated for biological control capability and systemic induction of resistance against the nematode m. javanica under glasshouse conditions....

A nematicide, beauvericin (1), was isolated from cultures of the fungus Fusarium bulbicola, and its structure was identified by spectroscopic analysis. Compound 1 showed nematicidal activities against the pine wood nematode Bursaphelenchus xylophilus and the free-living nematode Caenorhabditis elegans.

In-vitro methods were developed to test fungi for production of metabolites affecting nematode egg hatch and mobility of second-stage juveniles. Separate assays were developed for two nematodes: root-knot nematode (Meloidogyne incognita) and soybean cyst nematode (Heterodera glycines). For egg hatch to be successfully assayed, eggs must first be surface-disinfested to avoid the confounding effe...

Scanning electron micrographs of the nematode-egg-parasitic fungus Paecilomyces lilacinus infecting eggs of the root-knot nematode Meloidogyne spp. suggested the involvement of lytic enzymes. When grown on a liquid mineral salts medium, supplemented with different substrates as the sole N- and C-source, the fungus produced an extracellular protease. Colloidal chitin, vitellin and intact eggs of...

A high moisture level in the top 10 cm of soil at time of cutting of alfalfa increased the incidence of plant mortality and Fusarium wilt in soil infested with Ditylenchus dipsaci and Fusarium oxysporum f. sp. medicaginis in greenhouse and field microplot studies. Ranger alfalfa, susceptible to both D. dipsaci and F. oxysporum f. sp. medicaginis, was less persistent than Moapa 69 (nematode susc...

systemic induction of peroxidase and polyphenoloxidase in tomato roots and stem followed by application of split root system method was investigated. root system of tomato seedling in the sixth growth stage were divided in two equal sections after washing and replanted in two adjacent pots and then inoculated with nematode and fungus as fallowing treatments. the second treatment was inoculation...

over one-year period, 150 pasture soil samples and 138 sheep faecal samples, collected from different parts of iran were screened for the presence of nematophagous fungi. the samples were cultured at 25ºc on chloramphenicol-2% water agar (chf-wa) plates in the presence of haemonchus contortus third stage larvae (l3) and checked over a two-month period for characteristic conidia, conidiophores a...