نتایج جستجو برای: nested polymerase chain reaction nested

تعداد نتایج: 707924  

Journal: :Memorias do Instituto Oswaldo Cruz 2002
Pamela Cribb Juan Pablo Scapini Esteban Serra

Here we present a one-tube nested PCR test, which allows the detection of minimal quantities of Chlamydia trachomatis in human fluids. This assay includes the use of an internal control to avoid false negative results due to the presence of inhibitors. The results obtained show that this assay is robust enough to be used for clinical diagnosis.

2012
Chenga Tshering Mitsuhiro Takagi Eisaburo Deguchi

We detected Torque teno sus virus 1 and 2 (TTSuV1 and TTSuV2) in tissue samples from 18 stillborn piglets using nested polymerase chain reaction. The detection rates of TTSuV1 and TTSuV2 were 78% and 50%, respectively, with 83% of the stillborn piglets positive for TTSuV1 or TTSuV2. TTSuV1 was detected highest in the liver (72%) followed by heart (56%), spleen (38%) and tonsils (38%) while TTSu...

Journal: :Archives of disease in childhood. Fetal and neonatal edition 1999
B Fischler P Rodensjö A Nemeth M Forsgren I Lewensohn-Fuchs

AIM To time the onset of cytomegalovirus (CMV) infection in patients (n = 39) with CMV associated neonatal cholestasis by analysing CMV DNA on Guthrie cards sampled at 3 days of age. METHODS CMV infection was diagnosed by serology/urine isolation or by CMV DNA detection (polymerase chain reaction) in liver biopsy specimens. In order to time the infection dry blood filter paper discs were punc...

Journal: :Vector borne and zoonotic diseases 2012
Ana Vázquez María-Paz Sánchez-Seco Gustavo Palacios Francisca Molero Noelia Reyes Santiago Ruiz Carles Aranda Eduard Marqués Raul Escosa Juana Moreno Jordi Figuerola Antonio Tenorio

We report the characterization of three novel flaviviruses isolated in Spain. Marisma Mosquito virus, a novel mosquito borne virus, was isolated from Ochlerotatus caspius mosquitoes; Spanish Ochlerotatus flavivirus and Spanish Culex flavivirus, two novel insect flaviviruses, were isolated from Oc. caspius and Culex pipiens, respectively. During this investigation, we designed a sensitive RT-nes...

Journal: :Revista da Sociedade Brasileira de Medicina Tropical 2000
M T Cavasini W L Ribeiro F Kawamoto M U Ferreira

We have compared results of Plasmodium species identification obtained with conventional on-site microscopy of Giemsa-stained thick smears (GTS) and a semi-nested polymerase chain reaction (PCR) in 96 malaria patients from Rondônia, Western Brazilian Amazon. Mixed-species infections were detected by PCR in 30% patients, but no such case had been found on GTS. Moreover, P. malariae infections we...

2011
Emilie S. Zehr Louisa B. Tabatabai

A nested polymerase chain reaction (nPCR) assay was developed to determine the presence of a gene encoding a bacteriophage Mu-like portal protein, gp29, in 15 reference strains and 31 field isolates of Haemophilus parasuis. Specific primers, based on the gene’s sequence, were utilized. A majority of the virulent reference strains and field isolates tested harbored the gene. The results suggest ...

Journal: :Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc 2008
Dennis Kaw Gomez Gun Wook Baeck Ji Hyung Kim Casiano Hermopia Choresca Se Chang Park

Viral nervous necrosis (VNN) is a worldwide disease affecting several species of cultured marine fish. In Korea, VNN has been identified in several species of cultured marine fish. In this study, the authors present data of the amplified nested polymerase chain reaction product (420 bp) of 21 nodavirus strains from different species of apparently healthy wild marine fish on the southern coast o...

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