Suspension cultures of carrot (Daucus carota, line C1), tobacco (Nicotiana tabacum, line TX1), and Nicotiana plumbaginifolia (line NP) were frozen under controlled conditions with trehalose as the sole cryoprotectant. Maximal post-thaw viability (71-74%), measured by phenosafranin dye exclusion, was obtained with the C1 cells following a 24hour pretreatment with 5 or 10% trehalose and with 40% ...

Human interferon alpha2b gene was transiently expressed in Nicotiana excelsior plants. Fusion with N. plumbaginifolia calreticulin signal peptide for improved apoplast targeting and carrying out the expression under optimized conditions resulted in maximal interferon activity of 3.2 x 10(3) IU/g fresh weight (FW) with an average of 2.1 +/- 0.8 x 10(3) IU/g FW. It proves that N. excelsior is a s...

Healthy buds of Nicotiana plumbaginifolia growing in the Kashmir University Botanic Garden were selected for the present study. Flower development and senescence was divided into seven stages, viz., tight bud stage (I), mature bud stage (II), pencil stage (III), partially open stage (IV), open stage (V), partially senescent stage (VI) and senescent stage (VII). Various physiological and biochem...

HE origin of chromosome instability and subsequent chlorophyll variegation Tin hybrid derivatives of Nicotiana tabacum (n=24) and N . plumbaginifolia (n=10) has been described by MOAV (1961) and GUPTA (1965, 1968b). It was demonstrated by (GUPTA 1968b) that the alien chromosome of N . plumbaginifolia (pbg) carrying a Ws gene (chlorophyll factor) showed instability in association with the N . ta...

In eukaryotes, manganese superoxide dismutase is a nuclear-encoded protein that scavenges superoxide radicals in the mitochondrial matrix. We have isolated two manganese superoxide dismutase genes from Nicotiana plumbaginifolia L. and fused the 5' upstream regulatory region of these genes to the beta-glucuronidase reporter gene. The two gene fusions displayed a differential tissue specificity i...

Salinity is a major limiting factor for plant growth and development. To evaluate the impact of P5CS gene expression under in vitro salt stress condition, transgenic tobacco (Nicotiana plumbaginifolia) carrying P5CS gene and non-transgenic plants were treated with 0, 100, 150, 200 or 250 mM NaCl for 28 days. Proline content, lipid peroxidation and the activity of some antioxidant enzymes after ...