Tissue culturing, which is an alternative method to clonal reproduction of avocado (Persea americana Mill.) has started become widespread in recent years. However, the browning tissue resulting from oxidation phenolic compounds one most important factors limiting success vitro avocados. Therefore, this study, effects different antioxidants (activated charcoal (AC), ascorbic acid (ASA), citric (...

Micropropagation of Calophyllum brasiliense Cambess. (Clusiaceae) is a way to overcome difficulties in achieving large-scale plant production, given the recalcitrant nature of the seeds, irregular fructification and absence of natural vegetative propagation of the species. Cultures were established using nodal segments 2 cm in length, obtained from 1-2 year old seedlings, maintained in a greenh...

dendrobium chrysanthum (orchidaceae) is highly valued in the ornamental industry and has important medicinal properties used in the preparation of herbal medicines in different parts of the world. however, this orchid species is getting depleted from its natural habitats due to unsystematic collections. therefore, in the present study, an efficient mass propagation protocol through direct shoot...

Synthetic seed technology is an alternative to traditional micropropagation for production and delivery of cloned plantlets. Synthetic seeds were produced by encapsulating nodal segments of C. angustifolia in calcium alginate gel. 3% (w/v) sodium alginate and 100 mM CaCl2 · 2H2O were found most suitable for encapsulation of nodal segments. Synthetic seeds cultured on half strength Murashige and...

Cultures of whole fetal rat sensory ganglia which had matured and myelinated in culture were treated for 1-3 h with a pulse of 0.2% trypsin. The tissue was observed during the period of treatment and during subsequent weeks using both light and electron microscopy. Within minutes after trypsin addition the matrix of the culture was altered and the nerve fascicles loosened. Progressive changes i...

An efficient regeneration system was developed for Kigelia pinnata L., a multipurpose tree belonging to the family Bignoniaceae. The nodal segments were cultured in vitro, and the optimum concentrations of plant growth regulators for callus induction were determined. The friable organogenic calli were derived from the basal cut end of the nodal segments. The highest yield of morphogenic callus ...

Abstract Lavender plants are preferably propagated vegetatively. The aim of the work was to propose composition nutrient media for in vitro multiplication Lavandula angustifolia (var. Krajová and Beta) x intermedia Budrovka Grosso). Explant cultures were initiated from apical or nodal segments. Apical segments more suitable regenerated highest number shoots MS medium supplemented with 1 mg/L BA...

Context: The application of encapsulated shoot tips and nodal segments may contribute to the protection of rare and threatened medicinal plants. Although the artificial seed technique has been reported for more than two decades, for medicinal plants this method has not been developed sufficiently. The main limitations in conventional propagation of some species with medicinal value are: reduced...