OBJECTIVES To isolate a plant-derived compound with efflux inhibitory activity towards the NorA transporter of Staphylococcus aureus. METHODS Bioassay-guided isolation was used, with inhibition of ethidium bromide efflux via NorA as a guide. Characterization of activity was carried out using MIC determination and potentiation studies of a fluoroquinolone antibiotic in combination with the iso...

Short titles: Inverse Problems in Electrocardiology Address for correspondence: Dr. Robert S. Macleod, Nora Eccles Harrison CVRTI, Building 500, University of Utah, Salt Lake City, Utah. Telephone: (801)581-8183, FAX: (801)581-3128, Email: [email protected]. Support: Richard A. and Nora Eccles Harrison Treadwell Fund for Cardiovascular Research and awards from the Nora Eccles Treadwell Fou...

Die experimentelle Softwareentwicklungsumgebung NORA strebt die Nutzbarmachung neuer Ergebnisse im Bereich Unifikationstheorie und Deduktionsverfahren für Softwarewerkzeuge an. Gruppiert um eine Bibliothek wiederverwendbarer Softwarekomponenten bietet NORA interaktive Werkzeuge zum Komponentenretrieval mit Spezifikationen und Verwendungsmustern, zum unifikationsbasierten Konfigurationsmanagemen...

Nora virus is a single stranded RNA picorna-like virus with four open reading frames (ORFs). The coding potentials of the ORFs are not fully characterized, but ORF3 and ORF4 are believed to encode the capsid proteins (VP3, VP4a, VP4b, and VP4c) comprising the virion. To determine the polypeptide composition of Nora virus virions, polypeptides from purified virus were compared to polypeptides de...

We hypothesized that multidrug resistance efflux pumps (MDRs) may be contributing to the drug resistance of enterococci. We recently identified potential MDR-encoding genes in the Enterococcus faecalis V583 genome. Among the putative MDRs, we found a gene that encodes a NorA homolog and have characterized this enterococcal MDR in the present study. A mutant from which the enterococcal NorA homo...

RNA interference (RNAi) is a major antiviral pathway that shapes evolution of RNA viruses. We show here that Nora virus, a natural Drosophila pathogen, is both a target and suppressor of RNAi. We detected viral small RNAs with a signature of Dicer-2 dependent small interfering RNAs in Nora virus infected Drosophila. Furthermore, we demonstrate that the Nora virus VP1 protein contains RNAi suppr...

Drosophila melanogaster is widely used to decipher the innate immune system in response to various pathogens. The innate immune response towards persistent virus infections is among the least studied in this model system. We recently discovered a picorna-like virus, the Nora virus which gives rise to persistent and essentially symptom-free infections in Drosophila melanogaster. Here, we have us...