A screening method for methicillin-resistant Staphylococcus aureus (MRSA) by using selective broth and real-time PCR (broth-PCR) was developed and evaluated. The samples (n = 304) were cultured in the broth overnight, followed by nuc gene detection by real-time PCR. nuc-negative samples were further checked for the presence of nuc amplification inhibitors by a PCR internal inhibitor assay. nuc-...

A 3′-protected route toward the synthesis of diastereomers clinically active ProTides, NUC-1031 and NUC-3373, is described. The in vitro cytotoxic activities individual were found to be similar their diastereomeric mixtures. In KG1a cell line, NUC-3373 have preferential effects on leukemic stem cells (LSCs). These not diastereomer-specific observed with parental nucleoside analogues gemcitabine...

We describe a multiple dE/dx technique used to identify particles that penetrate through the bottom of the CRIS instrument, significantly extending the measured energy ranges for major elements beyond that for stopping particles. In preliminary analysis, the upper energy limit for oxygen has been extended from ∼240 MeV/nuc for stopping particles to ∼ 410 MeV/nuc for penetrating particles, and t...

We revisit the first-order approach to the moral hazard problem. Our fundamental claim is that in well-behaved problems, a condition we term No Upward Crossing (NUC) holds. We show how NUC facilitates analysis by both simplifying the relevant conditions for the validity of the first-order approach and giving them an economic interpretation. We provide extensive analysis of sufficient conditions...

Two genes, nuc-1 and nuc-2, of Neurospora crassa which were responsible for the nucleic acid digestion, were located on linkage group 1 and 2, respectively. A temperature-sensitive mutant (B1ts-2) was obtained from a nuc-2 mutant. Nuclease mutants showed a reduced activity of nuclease N(3) which was found to be a complex consisting of nuclease N(3) (') and inhibitor molecule. Nuclease N(3), nuc...

In wild-type Neurospora, alkaline phosphatase is made under conditions of phosphate limitation, but not conditions of phosphate sufficiency. Mutants at two unlinked loci, nuc-1 and nuc-2, do not make alkaline phosphatase under any conditions, while mutants at two quite closely linked loci, pcon and preg, make alkaline phosphatase even under conditions of phosphate sufficiency. pcon is extremely...

BACKGROUND Nucleos(t)ide analogues (NUCs) treatment can reduce the risk of hepatocellular carcinoma (HCC) development and recurrence in chronic hepatitis B (CHB) patients. However, the risk of recurrence in CHB patients who develop HCC despite NUC treatment remains unclear. METHODS 167 consecutive CHB patients receiving curative resection for HCC with NUC therapy after surgery were retrospect...

TCR signals induce the nuclear localization of NFATc proteins, which are removed from the nucleus after rephosphorylation by glycogen synthase kinase 3 and other kinases. Rapid nuclear export might allow continuous monitoring of receptor occupancy, making the transcriptional response proportional to the duration of TCR/CD28 signaling. To investigate this possibility, we analyzed mice in which T...

UNLABELLED Evolutionary conservation of the hypothalamus attests to its critical role in the control of fundamental behaviors. However, our knowledge of hypothalamic connections is incomplete, particularly for the lateral hypothalamic area (LHA). Here we present the results of neuronal pathway-tracing experiments to investigate connections of the LHA juxtaventromedial region, which is parceled ...

Let X be a Banach space with closed unit ball B. Given k ∈ N, X is said to be k-β, repectively, (k + 1)-nearly uniformly convex ((k + 1)-NUC), if for every ε > 0, there exists δ, 0 < δ < 1, so that for every x ∈ B, and every ε-separated sequence (xn) ⊆ B, there are indices (ni) k i=1, respectively, (ni) k+1 i=1 , such that 1 k+1 ‖x + ∑k i=1 xni‖ ≤ 1 − δ, respectively, 1 k+1 ‖ ∑k+1 i=1 xni‖ ≤ 1−...