Nucleic acids, especially RNA, naturally contain a diversity of chemically modified nucleosides. To understand the biological role of these modified nucleosides, nucleic acid scientists need tools to specifically label, detect and enrich modified nucleic acids. These tools comprise a diverse set of chemical reagents which have been established in the early years of nucleic acid research. Recent...

We describe a relatively inexpensive digitizing pad, available from Houston Instruments, for which we have written software which allows the direct reading of DNA (or RNA) sequencing gel autoradiographs. This eliminates several sources of error in nucleic acid sequencing, and renders computer analysis less cumbersome.

While initiation of transcription has attracted the most attention in the field of gene regulation, it has become clear that additional stages in the gene expression cascade including post-transcriptional events are under equally exquisite control. The seminal discovery that short RNAs (microRNA, small interfering RNA, Piwi-interacting RNA), play important roles in repressing gene expression ha...

introduction: p. aeruginosa is an important multi-drug resistant (mdr) opportunistic pathogen in hospital-acquired infections. several methods based on dna analysis have been used for investigating genetic diversity of p. aeruginosa isolates. among them, rep‑pcr typing has been introduced as a rapid, low cost method with high discriminatory power. the aims of this study were to investigate geno...

conclusions nucleic acid amplification tests (naats) are very appropriate in detection of infected individuals. detection techniques such as naats are independent of bacterial viability, and have a potential to limit false negative samples, therefore, in our country, the application of different laboratory diagnosis methods including naats with culture as gold standard for determination antimic...

Abstract Membrane‐spanning nanopores are used in label‐free single‐molecule sensing and next‐generation portable nucleic acid sequencing, as powerful research tools biology, biophysics, synthetic biology. Naturally occurring protein peptide pores, well inorganic nanopores, these applications, with their limitations. The structural functional repertoire of can be considerably expanded by functio...

ANALOG SOILS. A. Mojarro, J. Hachey, R. Bailey, M. Brown, R. Doebler, G. Ruvkun, M. T. Zuber, and C. E. Carr Department of Earth, Atmospheric and Planetary Science, Massachusetts Institute of Technology, 77 Massachusetts Ave, Cambridge, MA, 02139; Department of Molecular Biology, Massachusetts General Hospital, 185 Cambridge Street, Boston, MA, 02114; Claremont Biosolutions, 1182 Monte Vista Av...

High-throughput 454 DNA sequencing technology allows much faster and more cost-effective sequencing than traditional Sanger sequencing. However, the technology imposes inherent limitations on the number of samples that can be processed in parallel. Here we introduce parallel tagged sequencing (PTS), a simple, inexpensive and flexible barcoding technique that can be used for parallel sequencing ...