Rising levels of atmospheric CO2 are thought to increase C sinks in terrestrial ecosystems. The potential of these sinks to mitigate CO2 emissions, however, may be constrained by nutrients. By using metaanalysis, we found that elevated CO2 only causes accumulation of soil C when N is added at rates well above typical atmospheric N inputs. Similarly, elevated CO2 only enhances N2 fixation, the m...

Introduction Application of livestock manure provides nutrients for crop production and can also improve soil quality. However, the availability of nutrients in manure is difficult to predict. Therefore, livestock manure is frequently under or over-applied with respect to crop nitrogen and phosphorus requirements. In cases where the availability of nutrients is less than expected, application r...

A cost-effective protocol for uniform 15N and/or 13C isotope labeling of bacterially expressed proteins is presented. Unlike most standard protocols, cells are initially grown in a medium containing nutrients at natural abundance and isotopically labeled nutrients are only supplied at the later stages of growth and during protein expression. This permits the accumulation of a large cell mass wi...

Most dairy farms in Wisconsin continue to produce most of their feed and have sufficient land for recycling manure nutrients through crops. However, to remain economically viable, many dairy farms are increasing herd size and importing more feed. The increased importation of nutrients in the form of feed and fertilizer has resulted in excessive soil nutrient accumulation (Bundy, 1998; Proost, 1...

The effects of nutrients and planktivory on the accumulation of hydrophobic organic contaminants (HOCs) in aquatic food chains were investigated in large lake enclosures. Food-chain compositions in the enclosures were manipulated by additions of planktivorous fish (+F), nutrients (+N), both nutrients and fish (+NF), or received no additions (-NF). The treatments resulted in higher plankton but ...

For investigating the effect of vermicompost (VC) on improving phytoremediation of Pb by sunflower (Helianthus annuus) cultivar Lakumka, a factorial experiment was conducted in the pots containing soil or its mixture with 10% VC, in a greenhouse with controlled condition at 2018. After 3 weeks irrigation with normal water, plants were daily irrigated with high Pb-polluted water (0, 1000, 1500 o...