نتایج جستجو برای: off targeting
تعداد نتایج: 331126 فیلتر نتایج به سال:
Simultaneous multiplex mutation of large gene families using Cas9 has the potential to revolutionize agriculture and plant sciences. The targeting of multiple genomic sites at once raises concerns about the efficiency and specificity in targeting. The model Arabidopsis thaliana is widely used in basic plant research. Previous work has suggested that the Cas9 off-target rate in Arabidopsis is un...
cumin (cuminum cyminum) is an annual suitable for the dry and semi-dry areas of iran. recently, farmers have chosen to plant cumin because it requires less water and growth time. however, at harvest time, the resistance of cumin seed to shatter is low and, if careful attention is not paid to this fact, a major portion of the crop can be lost. when the connection force of the seed decreases, see...
Activation-induced cytidine deaminase (AID) initiates both somatic hypermutation (SHM) for antibody affinity maturation and DNA breakage for antibody class switch recombination (CSR) via transcription-dependent cytidine deamination of single-stranded DNA targets. Though largely specific for immunoglobulin genes, AID also acts on a limited set of off-targets, generating oncogenic translocations ...
Cas nucleases are popular tools for genome editing applications due to their ability to introduce DNA breaks at desired genomic locations. Such differential targeting is achieved through loading an RNA guide complimentary to the intended target sequence. As it turns out, sequences with only a partial match to the guide can also be cleaved. A large number of experiments have shed light on this o...
Synthetic 19 base pair (bp) RNA duplexes (small interfering RNAs, siRNAs) can enter RISC directly and induce RNAi-mediated cleavage of targeted transcripts without additional processing. Though this ability has enormous potential, expansion of RNAi technology requires overcoming obstacles associated with siRNA functionality and specificity. Initial problems associated with inconsistent gene kno...
multifunctional nanomaterials showed graet advantages in drug delivery. folic acid (fa) binding protein, a glycosyl phosphatidyl inositol anchored cell surface receptor for folate, is overexpressed in several human tumors, whereas it is highly restricted in normal tissues. therefore, in this study, fa, polyethylene glycol (peg), and fe3o4 nanoparticles multifunctionalized short multiwall carbon...
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