The modification of high-performance liquid chromatography parameters leads to a more effective oligonucleotide-A purification process. Using various experimental such as buffer, concentration, and pH, method for optimizing the an on reverse-phase C18 column was created. To purify oligonucleotide-A, High-Performance Liquid Chromatography (HPLC), Ultraviolet-Visible Spectrophotometry (UV-Vis), C...

The synthesis of a small number bis(imino)anthracene derivatives is reported. They were evaluated via NMR for binding efficacy to the G-quadruplex-forming oligonucleotide sequence (TTGGGTT) and show activity against HeLa cancer cell line.

UNLABELLED To investigate the feasibility of in vivo imaging study of atherosclerotic plaque and restenosis using antisense probe, we evaluated the uptake kinetics of radiolabeled oligonucleotides to the messenger RNA (mRNA) of proliferating cell nucleus antigen (PCNA) in vascular smooth muscle cells (VSMCs) and the effect on gene expression. METHODS The antisense oligonucleotide to PCNA was ...

Under the conditions normally used for detritylation in oligonucleotide synthesis, the haloacetic acid binds strongly to the oligonucleotide. Acetonitrile also forms a complex with the deblocking acid, in competition with the oligonucleotide, and drastically slows detritylation. Incomplete removal of acetonitrile during the deblock step may slow the kinetics enough to result in incomplete detri...

Here it was investigated how oligonucleotide retention and selectivity factors are affected by electrostatic non-electrostatic interactions in ion pair chromatography. A framework derived describing depends on the potential generated ion-pair reagent concentration, co-solvent volume fraction, charge difference between analytes, temperature. Isocratic experiments verified that, separation proble...

BACKGROUND Oligonucleotide-peptide conjugates have several applications, including their potential use as therapeutic agents. We developed a strategy for the chemical ligation of unprotected peptides to oligonucleotides in aqueous solution. The two compounds are joined via a stable amide bond in a template-directed reaction. RESULTS Peptides, ending in a carboxy-terminal thioester, were conve...

Here we report the preparation of poly(oligonucleotide) brush polymers and amphiphilic brush copolymers from nucleic acid monomers via graft-through polymerization. We describe the polymerization of PNA-norbornyl monomers to yield poly-PNA (poly(peptide nucleic acid)) via ring-opening metathesis polymerization (ROMP) with the initiator, (IMesH2)(C5H5N)2(Cl)2RuCHPh.1 In addition, we present the ...

The European Medicines Agency, the U.S. Food and Drug Administration, other regulatory agencies expect that all pharmaceutical products be assessed for potential presence of N-nitrosoamine (nitrosamine) impurities. This white paper addresses general considerations nitrosamine risk assessments oligonucleotide products. authors propose a assessment platform which should facilitate safe, consisten...