In heterothallic Ascomycota, two opposite but distinct mating types control all sexual processes. Using mating crosses, mating types were assigned to ten isolates of the heterothallic fungal species Ophiostoma quercus. Primers were subsequently designed to target the MAT1-1-1, MAT1-1-3 (of the mating type 1 idiomorph), and MAT1-2-1 (of the mating type 2 idiomorph) genes in these isolates. Resul...

Toxoplasma gondii infects all species of warm-blooded animals, including humans, and domestic cats and other felidae are its definitive hosts. The aim of the present survey was to compare serological and molecular tests in accurate diagnosis of T. gondii infection among stray cats in Ahvaz, Iran. A total of 100 cats were examined for the detection of serum antibodies against T. gondii (chronic ...

Aimed at investigating the recovery of a specific mutant allele of the mating type locus (MAT) by switching a defective MAT allele, these experiments provide information bearing on several models proposed for MAT interconversion in bakers yeast, Saccharomyces cerevisiae. Hybrids between heterothallic (ho) cells carrying a mutant MAT a allele, designated mata-2, and MAT alpha ho strains show a h...

Two mating-type genes, designated MAT1-1-1 and MAT1-2-1, were cloned and sequenced from the presumed asexual ascomycete Cladosporium fulvum (syn. Passalora fulva). The encoded products are highly homologous to mating-type proteins from members of the Mycosphaerellaceae, such as Mycosphaerella graminicola and Cercospora beticola. In addition, the two MAT idiomorphs of C. fulvum showed regions of...

background: this study is performed to reveal most common species and subspecies of leptospires that are main causes of hu­man leptospirosis in guilan, northern province of iran. methods: we performed igm-elisa and mat on 282 blood samples from patients who attended to 3 hospitals in the flat area of guilan province with clinical symptoms consisted with leptospirosis.  all specimens with titers...

background: toxoplasma gondii can infect all warm-blooded animals. modified agglutination test (mat) and elisa are widely used for the detection of t. gondii antibodies. however, there is little information on their acceptability for detecting antibodies in companion animals. methods: this study compared elisa and mat for their ability to detect t. gondii infection in natu­rally infected dogs a...