We have expressed recombinant RNase P protein from Escherichia coli in high yield. A hexahistidine sequence at the amino terminus allowed protein purification in a single step. Mass spectrometry confirmed the molecular weight of the purified protein and indicated a purity of > 95%. Protein functionality was demonstrated by reconstitution of active holoenzyme.

Site-specific fluorescent labeling of proteins in vivo remains one of the most powerful techniques for imaging complex processes in live cells. Although fluorescent proteins in many colors are useful tools for tracking expression and localization of fusion proteins in cells, these relatively large tags (>220 aa) can perturb protein folding, trafficking and function. Much smaller genetically enc...

BACKGROUND Making peptide pharmaceuticals involves challenging processes where many barriers, which include production and manufacture, need to be overcome. A non common but interesting research area is related to peptides with intracellular targets, which opens up new possibilities, allowing the modulation of processes occurring within the cell or interference with signaling pathways. However,...

Recombinant clathrin protein fragments form assemblies that template gold nanocrystals in an array across the latticed surface. The nanocrystals exhibit unusual anisotropic morphologies with long range ordering, both of which are dependent upon the presence of a hexahistidine tag on the clathrin heavy chain fragments.

The fact that Paul in a significant number of passages in his letters refers explicitly or implicitly to the Scriptures is taken as obvious evidence that he perceived Scriptures as authority not only for his own life but for the life of the Christ-followers he addresses as well. This perception of the Scriptures as the unquestioned authority at least in the life of Paul (and most likely the oth...

No positive authority Many people do not, or will not, appreciate that the mental welfare officer of today is not the duly authorised officer of the old legislation. Then he had direct responsibility and statutory authority to enable him to solve his problems. Under the Mental Health Act, he has less well defined responsibilities and no positive authority. Cases are still referred to him by all...

the sociology of law is concerned with explaining the relationship between law and society and studying of the social organization of law (as an order or a system), the social interactions of legal practitioners and people in this area and the meaning that people actually give to sociolegal realities. among the founding fathers of sociology, max weber is one of the most famous thinkers that eve...

Label-free detection of the interaction between hexahistidine-tagged human κ-opioid receptor membrane protein and anti-His antibody is demonstrated in liquid by an optical microelectromechanical system utilizing electromagnetically actuated microresonators. Shift in resonance frequency due to accretion of mass on the sensitive surface of microresonators is monitored via an integrated optical re...

We evaluate the ability of hexahistidine (His6) tags on peptide and protein substrates to recruit deoxyribozymes for modifying those substrates. For two different deoxyribozymes, one that creates tyrosine-RNA nucleopeptides and another that phosphorylates tyrosine side chains, we find substantial improvements in yield, kobs, and Km for peptide substrates due to recruiting by His6/Cu(2+). Howeve...