نتایج جستجو برای: organotypic culture
تعداد نتایج: 279099 فیلتر نتایج به سال:
The organotypic slice culture (Stoppini et al. A simple method for organotypic cultures of nervous tissue. 1991;37:173-182) has become the method of choice to answer a variety of questions in neuroscience. For many experiments, however, it would be beneficial to image or manipulate a slice culture repeatedly, for example, over the course of many days. We prepared organotypic slice cultures of t...
The specific activity of phosphatidate phosphohydrolase (PAPase) (EC 3.1.3.4) has been assayed in organotypic cultures of foetal rat lung type II alveolar pneumonocytes and in L2 cells derived from type II cells of the adult rat lung. This enzyme catalyses a critical step in the synthesis of phosphatidylcholine, the major lipid component of pulmonary surfactant. Surfactant is produced by the ma...
RATIONALE Cardiac tissue engineering should provide "realistic" in vitro heart muscle models and surrogate tissue for myocardial repair. For either application, engineered myocardium should display features of native myocardium, including terminal differentiation, organotypic maturation, and hypertrophic growth. OBJECTIVE To test the hypothesis that 3D-engineered heart tissue (EHT) culture su...
homocysteine thiolactone was demonstrated to inhibit the growth of 10–12-day-old chicken embryo cardiac tissue explants at 7 × 10−9 −1 × 10−3 m concentrations in a dose-dependent manner. the maximal cardiotoxic effect of homocysteine thiolactone was detected at 1 × 10−3 m, which corresponds to severe hyperhomocysteinemia. the results of experiments on culturing of cardiac tissue explants in the...
The behavior of embryonic murine lung cells on a basement membrane extract (Matrigel) was investigated. Single cell suspensions generated by trypsinization of lungs removed from day 12 embryos were plated on Matrigel and cultured for up to one week. The basement membrane extract was used as a gel, and as a wet or dried film. In all of these instances, organotypic arrangement of the embryonic lu...
The calyx of Held, a large axo-somatic relay synapse containing hundreds of presynaptic active zones, is possibly the largest nerve terminal in the mammalian CNS. Studying its initial growth in-vitro might provide insights into the specification of synaptic connection size in the developing brain. However, attempts to maintain calyces of Held in organotypic cultures have not been fruitful in pa...
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