Chloramphenicol (D-threo-2-dichloroacetamido-1-p-nitrophenylpropane-1,3-diol) added to blood samples did not alter the observed shift in the red cell osmotic fragility curves as the samples were aged in vitro for 24 hours at 37 degrees C. Nor was the normal rate of loss of 2,3-diphosphoglycerate, ATP, or glutathione from the red cells affected by the presence of chloramphenicol over the same pe...

The changes in the osmotic resistance of red blood cells (RBC), due to various pathological conditions like exposure to heat, anemia as well as the effect of storage and anticoagulants have been investigated. The results have shown that exposure to a temperature of 50 degrees C makes the red blood cells fragile to osmotic pressures. Also, red blood cells from the patients suffering from anemia ...

Cyclodextrins are used extensively in food, cosmetic, drug and chemical industries to increase aqueous solubility and stability, and reduce or eliminate the unpleasant taste and smell of many products. Experiments were conducted to examine the effect of a pre-haemolytic dose of methyl β-cyclodextrin (MBCD) on the osmotic fragility of erythrocytes collected from sheep, cattle and humans. Heparin...

The possibility that there is some common factor in protein deprivation and splenectomy affecting the properties of red cells was examined by measuring the lipid contents of the plasma and red cells, the surgace area and spherical index of red cells, and the lecithin cholesterol acyltransferase (LCAT) activity of the plasma in intact and splenectomized rats on diets with various levels of prote...

Road transportation and harmattan season have been reported to be stressful to live stock species. This study was conducted with the aim of investigating the effect of two and half hours of road transportation on the erythrocyte osmotic fragility of 23 healthy young adults Nigerians (15 males and 8 females) during the harmattan season. After an overnight fast, venous blood was collected from ea...

The effect of the sodium cyanate-induced carbamylation (carbamoylation) of proteins in erythrocytes was studied using spin labelling and spectrophotometric methods. The experiments were conducted in whole blood and in erythrocytes in phosphate buffer using 25 mmol/L of sodium cyanate. Lipid membrane fluidity was determined using three spin-labelled fatty acids: 5-, 12- and 16-doxylstearic acids...

SYNOPSIS Nineteen patients with morphological abnormalities of the red blood cells are described, and these formed approximately 3 % of the total cases of cardiac valvular disease. In two patients the abnormal blood film developed after the insertion of an aortic and mitral valve prosthesis respectively, but in another two patients the abnormal blood film was corrected by aortic valve surgery. ...

By DAVID \V. \\ESTRING AND SUZANNE B. BnrrrlN T lIE HEMOLYTIC RESPONSE of erythrocytes to hypotonic stress is well known. Complex enzyme systems are required by the cell to maintam membrane integrity during exposure to hypotonic solutions. When certain of these enzymes are defective, erythrocyte osmotic fragility may be altered.3 On the other hand, the response of leukocytes to osmotic stress h...