Highly reactive species at small copy numbers play an important role in many biological reaction networks. We have described previously how these species can be removed from reaction networks using stochastic quasi-steady-state singular perturbation analysis (sQSPA). In this paper we apply sQSPA to three published biological models: the pap operon regulation, a biochemical oscillator, and an in...

Detection of azide-tagged biomolecules (e.g., azido sugars) inside living cells using “click” chemistry has been revolutionary to the field chemical biology. However, we currently still lack suitable synthetic biology tools autonomously and rapidly detect azide ions. Here, have developed an engineered promoter system called cyn regulon, complementary Escherichia coli strains, selectively ions i...

background: phosphatidate phosphohydrolase (pap) catalyzes the dephosphorylation of phosphatidic acid to yield p i and diacylglycerol. two different forms of pap have been reported in rat hepatocyte: pap 1 that participates in the synthesis of phospholipids and triacylglycerols and pap 2 which is involved in lipid signaling pathways. two isoforms of pap 2 are pap 2a and pap 2b . this study exam...

Fractional flow reserve (FFR) is considered the standard for assessment of physiological significance coronary artery stenosis. Intracoronary papaverine (PAP) most potent vasodilator used achievement maximal hyperemia. However, its use can provoke ventricular tachycardia (VT) due to excessive QT prolongation. We evaluated clinical efficacy and safety administration PAP after nicorandil (NIC), a...

objective(s): in developing countries and worldwide cervical cancer is an important cause of   female mortality. reports describing the frequency and pattern of abnormal pap smears in saudi arabia, using the revised bethesda system (rbs) are very few. the current study was conducted to explore the changing pattern of epithelial cell abnormalities (eca) detected in pap smears (ps) in females of ...

objective(s): the major antibiotic efflux pump of esherichia coli is acrab-tolc. the first part of the pump, acrab, is encoded by acrab operon. the expression of this operon can be kept elevated by overexpression of an activator, mara following inactivation of marr and acrr repressors due to mutation in encoding genes, marr and acrr, respectively. the aims of this research were to use e. coli m...