The use of DNA technology for parentage testing is increasing every day. Most laboratories have improved their exclusion probabilities (PE) by the addition of DNA microsatellite loci to standard blood-typing results. The efficacy of each locus depends on the number of alleles detected and their frequencies in the breeds tested. Here we analyzed the usefulness of six microsatellites for routine ...

Santa Inês is the most common hair sheep breed in Brazil and probably has the highest genetic diversity among sheep breeds in this country. Successful breeding programs for Brazilian sheep breeds are not common for various reasons, including a lack of control of parentage in the flocks. We developed an allele frequency database for 23 STR loci for the Santa Inês breed based on 285 animals ...

The present study was to construct a parentage testing system for Thoroughbred (TB) horse. A total number of 1,285 TB horse samples including 962 foals for parentage testing, 9 sires and 314 dams for individual identification were genotyped. Genomic DNA was extracted from 5 hair roots and genotyped by using 14 microsatellite markers (AHT4, AHT5, ASB2, ASB17, ASB23, CA425, HMS1, HMS3, HMS6, HMS7...

for identification of individuals and parentage control performed by cattle breeders in the czech republic, a novel finnish bovine genotypes™ panel 3.1was amplified by means of one multiplex polymerase chain reaction. bovine panel encompasses all the 12 str loci recommended by the international society for animal genetics (isag) for routine use in parentage testing and identification, including...

A set of 233 Holstein calves, their 233 dams and 44 sires from 4 large-sized herds was genotyped for 10 microsatellites recommended by ISAG for paternity/parentage testing. Heterozygosity of microsatellites ranged from 0.607 (SPS115) to 0.835 (TGLA227), and PIC from 0.575 (SPS115) to 0.816 (TGLA227) confirming a high polymorphism of each analysed locus. Their combined exclusion probability reac...

We propose the use of single nucleotide polymorphisms (SNPs) instead of polymorphic microsatellite markers for individual identification and parentage control in cattle. To this end, we present an initial set of 37 SNP markers together with a gender-specific SNP for identity control and parentage testing in the Holstein, Fleckvieh and Braunvieh breeds. To obtain suitable SNPs, a total of 91.13 ...