Research has demonstrated that swine feed can be a fomite for viral transmission and additives reduce contamination. Therefore, the objective of this study was to evaluate two in contaminated with PEDV or PRRSV. Feed included: no treatment, 0.33% commercial formaldehyde-based product, 0.50% medium chain fatty acids (MCFA) blend. samples were inoculated PRRSV alone together at an inoculation con...

Many applications in molecular biology can benefit from improved PCR amplification of DNA segments containing a wide range of GC content. Conventional PCR amplification of DNA sequences with regions of GC less than 30%, or higher than 70%, is complex due to secondary structures that block the DNA polymerase as well as mispriming and mis-annealing of the DNA. This complexity will often generate ...

A calibrated pipettor may be 99% accurate and precise, but tips can compromise such accuracy and precision by binding sample. Low binding tips improve accuracy and precision by utilizing Bonded Polymer Technology to bond a proprietary polymer to the tip resin. This advanced process creates a hydrophobic surface that reduces the surface tension of the interior wall of the pipette tip, resulting ...

Olmesartan medoxamil (OLM, an angiotensin II receptor blocker), amlodipine besylate (AML, a dihydropyridine calcium channel blocker) and hydrochlorothiazide (HCT, a diuretic of the class of benzothiadiazines) are co-formulated in a single-dose combination for the treatment of hypertensive patients whose blood pressure is not adequately controlled on either component monotherapy. In this work, t...

In molecular biology, polymerase chain reaction (PCR) has played an important role but suffers a general problem of low efficiency and specificity. Development of suitable PCR additives to improve the specificity and efficiency still remains a great challenge. Here we report the use of dendrimer-entrapped gold nanoparticles (Au DENPs) as a novel class of enhancers to improve the specificity and...

There is a general necessity to improve the specificity and efficiency of the polymerase chain reaction (PCR), and exploring the PCR-enhancing mechanism still remains a great challenge. In this paper we report the use of branched polyethyleneimine (PEI)-based derivatives and hybrid nanocomposites as a novel class of enhancers to improve the specificity and efficiency of a nonspecific PCR system...

Introduction: Researchers have recently described a relationship between food additives and immune system responses. A decline in immune response has also been observed in people who are in contact with food additives, such as preservatives and flavours found in different foods. Objectives: The objective of the present study was to investigate the in vitro effect of food additives, such as vani...

This paper reviews the use of additives to reduce odor and ammonia (NH3) emissions from livestock wastes. Reduction of NH3 volatilization has been shown to be possible, particularly with acidifying and adsorbent additives, and potential exists to develop further practical and cost-effective additives in this area. Masking, disinfecting, and oxidizing agents can provide short-term control of mal...