نتایج جستجو برای: pcr assay to detect the genes encoding sea
تعداد نتایج: 17495683 فیلتر نتایج به سال:
abstract the present study was conducted in order to investigate the impact of an integrated model of form-focused and task-based instruction on iranian efl learners vocabulary learning and retention.it also aimed to detect efl learners attitude towards the implementation of form-focused task-based vocabulary instruction in the classroom. in order to address the purposes of this study, a sampl...
Background and Objective: Despite the critical importance of catheter as an indwelling medical device, its prolonged utilization in hospitalized patients may lead to infection. This study aimed to identify distribution of uropathogenic bacteria isolated from catheterized uro-oncology patients, their biofilm production, and antimicrobial resistance patterns to generally -used antibiotics. Mater...
conclusions prevalence of icaadbc genes was relatively high among children and also all the four ica genes were detected among mrsa strains. results five isolates (22.7%) were methicillin resistant (mrsa) and meca gene was detected among them. all the mrsa isolates harbored sccmec type iii. prevalence of icaa, icab, icac and icad in the isolates were 16 (73%), 14 (63.6%), 16 (73%) and 16 (73%),...
background : infections resulted from multi-resistant staphylococcus aureus are increasing worldwide. in the present study, a multiplex-pcr assay for the detection of antibiotic resistance genes among s. aureus clinical isolates and for the concomitant identification of these isolates was described. methods : a total of 127 s. aureus isolates were collected from clinical specimens at three teac...
background escherichia coli o157:h7 is an enteric pathogen which can be frequently found asymptomatically in ruminant mammals, but can cause diseases from mild diarrhea to hemolytic uremic syndrome in humans. methods we developed fluorescent amplification-based specific hybridization (flash–pcr) assay to detect the stx-encoding gene stx-1 of e. coli o157:h7. result pcr product of 336 bp was suc...
Introduction & Objective: Fluoroquinolones are essential antimicrobial agents used to treat UTIs. Clinical experiences have shown a high rate of antibiotic resistance among uropathogens. These resistance are usually the consequence of mutations involving genes encoding gyrA and parC. The aim of this study was to determine antimicrobial resistance pattern and the presence of mutations in regions...
Background and Aims: Staphylococcus aureus (S. aureus) is considered as one of the most dangerous pathogenic bacteria due to the production of extracellular toxins. The objective of this study was to determine the prevalence of S. aureus and to characterize the recovered strains for their enterotoxin-producing genes in raw cow milk. Materials and Methods: During 9 months duration of the s...
ABSTRACT- Today, the authenticity of meat products with less costly and desirable species has increased. Therefore and considering religious, economicalor public health concerns, proper actions should be taken to prevent such frauds. In this study, real time PCR assay was applied for rapid, sensitive and specific identification and quantification of chicken tissue in meat products. Specific pri...
Background: The virulence of a pathogenic Vibrio cholerae is dependent on a discrete set of genetic determinants. In this study, we determined the distribution of virulence determinants among the clinical and environmental isolates of V. cholerae. Methods: The antibiotic resistance profiles of the isolates were determined using standard disk diffusion assay. PCR assay was performed to analyze t...
Introduction: Methicillin-resistant Staphylococcus aureus (MRSA) is an important pathogen that causes several nosocomial or community-acquired infections. Adhesion to surfaces and subsequent biofilm formation are the major phases of a staphylococcal infection. The aim of this study was to detect the presence of icaABCD genes in clinical isolates of MRSA. Methods: A total of 110 clinical Staphyl...
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