6-Aminopenicillanic acid may be quantitatively estimated by its reaction with fluorescamine in the concentration range of 1 to 10 micrograms/ml. The difference in reactivity between 6-aminopenicillanic acid and benzylpenicillin, which does not react with fluorescamine, can be used to determine penicillin acylase activity and obtain data on enzyme parameters and inhibitors. Unlike amino acids an...

Penicillin acylase, an industrially important biocatalyst catalyzes the conversion of penicillins to 6-amino penicillanic acid (6-APA) which is the main precursor for the production of semi-synthetic -lactam antibiotics. The present work involves the continuous production of 6-APA in a packed column reactor by using agarose immobilized penicillin acylase as a block polymer. The strain Escherich...

Kinetic experiments with a substrate series of phenylacetyl-arylamides reveal that at least one polar group in the amine moiety is required for the proper orientation of the substrate in the large nucleophile-binding subsite of penicillin acylase of Escherichia coli. Quantum mechanical molecular modelling of enzyme-substrate interactions in the enzyme active site shows that in the case of subst...

we introduce a new smooth, non-toxic, biocompatible method for cross-linking of gum tragacanth (gt), a polysaccharide of natural origin, in order to serve as a new supporting matrix for immobilization systems.  the modified gum is used as a matrix for the catalysis of the conversion of benzyl penicillin to 6-aminopenicillanic acid (6-apa) by means of escherichia coli atcc11105 with penicillin g...

A new enzymatic process is reported, in which penicillin G acylase from Escherichia coli displays a promiscuous activity in catalyzing the Markovnikov addition of allopurinol to vinyl ester.

The large heterodimeric penicillin G acylase from Alcaligenes faecalis was displayed on the surface of phage fd. We fused the coding sequence (alpha subunit-internal peptide-beta subunit) to the gene of a phage coat protein. A modified g3p signal sequence was used to direct the polypeptide to the periplasm. Here we show that a heterodimeric enzyme can be expressed as a fusion protein that matur...

We designed and prepared nanoreactors based on a poly(2-methyloxazoline)-block-poly(dimethylsiloxane)-block-poly(2-methyloxazoline (PMOXA-b-PDMS-b-PMOXA) amphiphilic triblock copolymer encapsulating the enzyme penicillin acylase for local and controlled production of antibiotics.

Aculeacin A acylase from Actinoplanes utahensis produced by Streptomyces lividans 1 revealed new acylase activities being able to hydrolyse penicillin V and several natural 2 aliphatic penicillins. Penicillin K was the best substrate showing a catalytic efficiency of 3 34.79 mM-1 s-1. Furthermore, aculeacin A acylase was highly thermostable with a midpoint 4 transition temperature (Tm) of 81.5º...

Penicillin acylases (PA) are widely used for the production of semi-synthetic β-lactam antibiotics and chiral compounds. In this review, the latest achievements in the production of recombinant enzymes are discussed, as well as the results of PA type G protein engineering.