A dimeric cyclic cysteine analogue, i. e. (1R,1'R,2R,2'R)-2,2'-disulfanediylbis (aminocyclohexane-1-carboxylic acid), was used as a constrained unnatural amino acid and folding inducer in ultra-short Leu-Val-containing peptide. Our results showed that both free dimer L1 its peptide derivative L2 are able to chelate Cu(II). The obtained complexes resulted be catalytically active Michael addition...

Lasso peptides form a class of ribosomally synthesized and post-translationally modified (RiPPs) characterized by mechanically interlocked topology, where the C-terminal tail peptide is threaded trapped within an N-terminal macrolactam ring. Sphingonodin I lasso that has not yet been structurally using traditional structural biology tools (e.g., NMR X-ray crystallography), its biological functi...

Peptides corresponding to the immunodominant loop located at residues 135-158 on capsid protein VP1 of foot-and-mouth disease virus (FMDV) generally elicit high levels of anti-peptide and virus-neutralizing antibodies. In some instances, however, the level of neutralizing antibodies is low or even negligible, even though the level of anti-peptide antibodies is high. We have shown previously tha...

BACKGROUND The Resonant Recognition Model (RRM) is a physico-mathematical model that interprets protein sequence linear information using digital signal processing methods. In this study the RRM concept was employed for structure-function analysis of myxoma virus (MV) proteins and the design of a short bioactive therapeutic peptide with MV-like antitumor/cytotoxic activity. METHODOLOGY/PRINCI...

Macropinocytosis is a ubiquitous cellular uptake mechanism of peptide-based intracellular delivery. This entry pathway shows promise as route for the biomacromolecules and nanoparticles. In this work, we obtained 8-residue analogue P4A bearing higher macropinocytosis induction ability. contains vital cysteine residues in its sequence, which immediately reacts with cystine culture medium to conv...

The chemical structure of oligonucleotide analogues dictates the conformation analogue oligomers, their ability to hybridize complementary DNA and RNA, stability degradation pharmacokinetic properties. In a study aimed at investigating new featuring neutral backbone, we explored oligomers containing morpholino-peptide backbone bind oligonucleotides. Circular Dichroism studies revealed our inter...

Th0 clones recognizing an immunodominant peptide of myelin basic protein (residues 83-99) were derived from patients with multiple sclerosis. We demonstrate that analogue peptides with alanine substitution at Val86 and His88 had a unique partial agonistic property in inducing Th0 -->Th1 and Th0 -->Th2 deviation of the myelin basic protein-reactive T cell clones, respectively. Th0 to Th1 deviati...

A recent study showed that the novel pituitary adenylate cyclase-activating peptide (PACAP)-27 analogue [Arg15,20,21,Leu17]-PACAP-27-Gly-Lys-Arg-NH2 causes sustained airway smooth muscle relaxation in vitro. This study examined whether this analogue also has bronchoprotective effects, by inhibiting muscarinic airway responsiveness in vivo. Total lung resistance was measured in anaesthetized, tr...

We have previously identified the novel Cancer/Testis antigen PASD1 by immunoscreening a testis library with pooled acute myeloid leukemia (AML) patient sera. To develop a cytotoxic T lymphocyte (CTL)-inducing vaccine, we have now investigated the carboxy-terminal region, known to contain serological determinants, for MHC class I (HLA-A*0201)-binding peptides. Algorithmselected natural peptides...

The contact system comprises a series of serine proteases that mediate procoagulant and proinflammatory activities via the intrinsic pathway coagulation kallikrein–kinin system, respectively. Inhibition Factor XIIa (FXIIa), an initiator has been demonstrated to lead thrombo-protection anti-inflammatory effects in animal models serves as potentially safer target for development antithrombotics. ...