Directed mutagenesis of a gene coding for a membrane protein of the periodontopathogen Actinobacillus actinomycetemcomitans was achieved by conjugation. The gene was disrupted by insertion of an antibiotic cassette into a unique endonuclease restriction sequence engineered by inverse PCR. The disrupted gene was cloned into a conjugative plasmid and transferred from Escherichia coli to A. actino...

The asaccharolytic periodontopathogen Porphyromonas gingivalis produces membrane-anchored proteases such as dipeptidyl peptidase IV that are involved in the destruction of host periodontal tissue. The extracellular domain of this enzyme was overexpressed in Escherichia coli as an N-terminal His-tag fusion protein, purified using standard metal-affinity chromatography and crystallized using the ...

Phagocytosis and killing of pathogens by polymorphonuclear neutrophils (PMN) from gingival crevicular fluid (GCF) is diminished in chronic periodontitis patients. As an approach to improve targeting of PMN toward a periodontopathogen, Porphyromonas gingivalis, the efficacy of a bispecific antibody (BsAb) directed against both recombinant 130 kDa hemagglutinin domain (r130k-HMGD) of P. gingivali...

A massive periodontal destruction can affect the root canal (RC) system and potentially expose pulpo-dentinal complex to opportunistic bacteria. Streptococcus mutans is a major pathogen of human caries diseases, its virulence mostly resides in ability adhere collagen form biofilms, due collagen-binding proteins (CBPs) Cnm Cbm. Seventeen patients affected by severe endo-periodontal lesions witho...

Periodontal disease is the major cause of tooth loss in adults. Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans are considered key pathogens in periodontitis. The treatment consists of oral hygiene education, instrumentation for removal of calculus (scaling), chemotherapy and periodontal surgery. Several agents are commercially available; these chemicals can alter oral microb...

Actinobacillus actinomycetemcomitans SUNY 465, the invasion prototype strain, enters epithelial cells by an actin-dependent mechanism, escapes from the host cell vacuole, and spreads intracellularly and to adjacent epithelial cells via intercellular protrusions. Internalized organisms also egress from host cells into the assay medium via protrusions that are associated with just a single epithe...

Pendahuluan: Resistensi antimikroba konvensional pada bakteri patogen merupakan masalah kesehatan dan ekonomi masyarakat, maka perlu adanya alternative dalam menangani infeksi. Antimikroba peptide kationik Human beta defensin -3 (HBD-3) yang dihasilkan epitel gingiva,secara alamiah ditemukan di sistem imun tubuh manusia antibakteri spektrum luas jarang menimbulkan resistensi serta alergi. Aktiv...