results of 45 patients, 4 (8.9% [95% ci: 4.4-15.6]) had detectable genomic hcv-rna in their pbmc specimens. hcv genotypes were determined in the pbmcs of these subjects showed that 2 (50.0%) subjects with occult hcv infection had hcv subtype 3a, and 2 (50.0%) had hcv subtype 1b. conclusions this study found that 8.9 % of the iranian candidates for liver transplant with cryptogenic cirrhosis had...

A growing number of autoimmune diseases, allergies, and atopic conditions in recent years are calling for the development innovative therapies, such as those using stem cells with immunoregulatory activity. Because few often conflicting results scientific literature, it is still uncertain whether mesenchymal (MSC) could be a useful therapeutic strategy targeting B lymphocytes helpful treating a...

Factors that influence viability and function of cryopreserved peripheral blood mononuclear cells (PBMC) were identified on 54 samples from 27 AIDS Clinical Trial Units. PBMC viability ranged from 1 to 96% with a median of 70%, was higher in laboratories with experienced staff, and was not significantly associated with CD4 cell number. Function of cryopreserved PBMC, measured by lymphocyte prol...

background: there is a worldwide interest in searching for potential and effective medicinal plant candidates against various diseases or disorders. till now, there are number of anti-inflammatory drugs like nsaids are available which showed various adverse effects in our body. to reduce these adverse effects, some of medicinal plants have been experimentally validated. aim: the aim of our immu...

Abstract Human immune cell reconstitution (Peripheral Blood Mononuclear Cells; PBMC or CD34 +hematopoietic stem cell; HSC) is commonly performed in immunodeficient mouse strains, such as NCG, NSG, NOG. utilizing PBMCs a much more rapid model, allowing for T-cell within 2 weeks. Due to the occurrence of xenogeneic graft-versus-host disease (xGvHD) around 4 weeks post-engrafment, application PBMC...

Objective: ABCG8 plays a crucial role in the reverse transfer of cholesterol from macrophages to the liver. The herbal compliments and regular physical improve the lipid profile and metabolic disorders. The aim of this study was to evaluate the effect of eight weeks aerobic exercise and green tea on the ABCG8 gene expression of peripheral blood mononuclear cell (PBMC) and the atherogenic ratio ...

Inflammation is common to many disorders and responsible for tissue and organ damage. In many disorders, the associated peripheral cytokine milieu is dilute and difficult to measure, necessitating development of more sensitive and informative biomarkers for mechanistic studies, earlier diagnosis, and monitoring therapeutic interventions. Previously, we have shown that plasma of recent-onset (RO...