The intracellular site of synthesis of two peroxisomal enzymes of rat liver, uricase (urate:oxygen oxidoreductase, EC 1.7.3.3) and catalase (hydrogen peroxide:hydrogen peroxide oxidoreductase, EC 1.11.1.6), has been localized on free ribosomes and not membrane-bound ribosomes. Free polysomes and membrane-bound polysomes, prepared by classical cell fractionation techniques from rat liver, were i...

OBJECTIVE The aim of this study was to examine the in vitro effect of peroxisomal dysfunction on lysosomal enzymes, the autophagic machinery in the cell, in order to understand the mechanisms of pathogenesis of peroxisomal disorders. MATERIALS AND METHODS Foreskin samples were obtained immediately after circumcision of 1- to 2-day-old infants at the Maternity Hospital, Kuwait. Skin tissues we...

Is Malondialdehyde a Putative Uremic Toxin? B.K. Kishore B.K. Kishore, MD, BCHM 75.39 ICP/UCL, avenue Hippocrate 75, B-1200 Bruxelles (Belgium) Dear Sir, References Various studies have demonstrated that the lipid per-oxidation (LP) status of chronic renal failure (CRF) patients is significantly increased, irrespective of the dialysis treatment [1–3]. While the actual cause for this increased L...

Copperand zinc-containing Superoxide dismutase, man ganese-containing Superoxide dismutase, catalase, and glutathione peroxidase form the primary enzymic defense against toxic oxygen reduction metabolites. Such metabolites have been implicated in the damage brought about by ionizing radiation, as well as in the effects of several cytostatic com pounds. These enzymes were analyzed in 31 differen...

In a recent article in this journal (1), a limited number of papers dealing with variations of malondialdehyde (MDA) in different pathological conditions were reviewed. After a brief introduction on the importance of oxidative stress, MDA production pathways, and analytical techniques, Singh et al. (1) concluded as " MDA is a useful bi-omarker for lipid peroxidation and oxidative stress. " The ...

whiclh will eventually be publislhed under a single cover. There are eight contributions to the present volume, whiclh provide useful summaries of the work of the various participants. The first paper, "Evidence for a membrane-linked Ca++ carrier in rat liver andI kidlney mitoclhondria," by A. L. Lehninger and E. Carafoli, is unrelated to the others. It concerns the dynamics of Ca++ movements c...

JAYLI:’ foiiiid that oti addition of a si ttI1 ai ount of hen og1ohin, rtiin shows weak peroxidase a(tivity with au optiintii around pH 4.4 in sJ)itP of the fact that no 50(11 a(1ivitv ean })e dei oiistiated in either suI)stan(e hv itS( 1f. In later investigations iii association with POlOliOVski he (lemonstrated that a complex l)et\VN9I hemoglobin (Jib) an(l a serum protein is responsible for ...

an organism are compared. In mammals, for example, the ER is vastly abundant in exocrine pancreatic secre-where there is no appreciable demand for the secretion of any protein (Bolender, 1974). Using representative examples primarily from the yeast Saccharomyces cere-One hallmark of eukaryotic cells is that they contain visiae, we highlight in this review some recent insights many functionally ...

Nifurtimox, is a nitrofuran compound with a trypanocidal effect against both trypomastigote and amastigote forms of T. cruzi. Its antiparasitic activity is due to the formation of toxic derivatives of oxygen when nifurtimox is reduced. T. cruzi presents low levels of reduced glutathione and lacks catalase and glutathione peroxi-dase, which makes the parasite very sensitive to hydrogen perox-ide...

al., 2002). Cytotoxicity caused by ROS is mediated in part by the JNK pathway (Matsuzawa et al., 2002). Acti-During inflammation, NF-␬B transcription factors an-vation of this pathway by TNF␣ promotes death by in-tagonize apoptosis induced by tumor necrosis factor ducing cleavage of the Bcl-2 family BH3-only protein, (TNF)␣. This antiapoptotic activity of NF-␬B involves Bid, into jBid, which ta...