Previous studies have reported that myosin can be modified by oxidative stress and particularly by activated haem proteins. These reactions have been implicated in changes in the properties of this protein in food samples (changes in meat tenderness and palatability), in human physiology (alteration of myocyte function and force generation) and in disease (e.g. cardiomyopathy, chronic heart fai...

Background Lytic polysaccharide monooxygenases (LPMO) release a spectrum of cleavage products from their polymeric substrates cellulose, hemicellulose, or chitin. The correct identification and quantitation of these released products is the basis of MS/HPLC-based detection methods for LPMO activity. The duration, effort, and intricate analysis allow only specialized laboratories to measure LPMO...

white-rot basidiomycetes are one of the most important lignolytic microorganisms. these fungi have been reported to secrete three main classes of lignin degrading enzymes: lignin peroxidases (lips), manganese peroxidases (mnps) and laccases. in this study, for the first time the lignin degrading capability of two plant pathogens i.e. hyphodermella rosae and h. corrugata was evaluated using both...

Residues of several crops, including wheat, have a promising allelopathic effect on noxious weed species and thus represent eco-friendly alternatives to harmful, widely applied herbicides. The current investigation deals with the effects wheat straw aqueous extract growth biochemical aspects bermudagrass (Cynodon dactylon L.) as model harmful weeds for crop. prepared from was subjected high-per...

BACKGROUND Plants are an efficient source of natural antioxidant against free radicals causing kidney damages. Sida cordata ethyl acetate fraction has been reported for strong in vitro antioxidant potency, previously. In the present study, our objective was to evaluate its in vivo antioxidant potency against CCl4 induced nephrotoxicity and investigates the bioactive phytochemicals by HPLC-DAD a...

Effect of drought stress induced by mannitol on secondary products in callus Golden Sunrise cherry tomatoes were studied vitro. Seeds irradiated with gamma at the doses (0, 20, or 40 Gy), and germinated Murashige Skoog, (MS) basal medium. Callus initiated from cotyledon leaf explants using a combination (2.0 kin + 2.0 IAA mg. l-1) then transferred into MS medium supplemented concentrations 40, ...

Bacterial-fungal interactions are important in the functioning of natural ecosystems. We examined possible synergistic or antagonistic effects during degradation polycyclic aromatic hydrocarbons (PAHs) by a fungal–bacterial co-culture. Bacteria and fungi were grown liquid nutrient medium supplemented with PAH substrates. The PAHs identification metabolites checked HPLC. Enzyme activities spectr...

background: elicitors can trigger defense responses and activate specific genes involved in secondary metabolite biosynthesis. objective: for understanding sa-treatment signaling pathway we determined defense-related compounds and enzymes in s. marianum hairy root cultures. methods: 28 day old hairy roots were treated by salicylic acid (6 mg/50 ml culture) for different times (24, 48, 72, 96 an...

Human glutathione transferases (GSTs) from Alpha (A), Mu (M) and Theta (T) classes exhibited glutathione peroxidase activity towards phospholipid hydroperoxide. The specific activities are in the order: GST A1-1>GST T1-1>GST M1-1>GST A2-2>GST A4-4. Using a specific and sensitive HPLC method, specific activities towards the phospholipid hydroperoxide,1-palmitoyl-2-(13-hydroper oxy-cis-9, trans-1...