Quantitative analysis of triacylglycerols (TGs) in plant oils and animal fats by normalization of peak areas can lead to erroneous results due to the large response differences with common HPLC detectors between the various TGs. The charged aerosol detector (CAD), that generates an almost universal response for non-volatile compounds, was combined with non-aqueous reversed-phase HPLC (NARP-HPLC...

gc-fid and hplc-dad were developed for determination of menadione (mn) and menadione sodium bisulphite (msb). by means of each method, quantitative analysis of msb in commercial pharmaceutical was performed in both direct analysis of msb and analysis of mn by converting msb to mn with sodium carbonate. gc-fid method was carried out on the hp-5 capillary column using nitrogen gas. hplc-dad metho...

A high-performance liquid chromatography with ultraviolet detection (HPLC/UV) methodwhich was used solid-phase extraction for cleanup, was developed for the determination ofAzinphos methyl ,Phosalone, Diazinon and Chlorpyrifos residues in fruits. A full factorialexperimental design was used for development of HPLC condition and evaluation of effect ofthree factors (pH of mobile phase, percent o...

We here report a reversed-phase HPLC method for the determination of free cortisol in human urine, using methylprednisolone as the internal standard. Before chromatography, samples were extracted with a C18 solid-phase extraction column and the steroids were separated on a LiChrospher 100 C18 column with a mobile phase of methanol/acetonitrile/water (43/3/54 by vol). Linearity, precision, and a...

BACKGROUND Size-exclusion HPLC has been used as an alternative to immunoassays for quantifying urinary albumin (microalbumin). Systematically higher values for the HPLC method have been proposed to result from nonimmunoreactive albumin. METHODS We evaluated separation of purified proteins and urinary components by size-exclusion HPLC using a Zorbax Bio Series GF-250 column eluted with phospha...

A solid-phase extraction (SPE) method for sample clean-up and preconcentration followed by reversed-phase high-performance liquid chromatography (RP-HPLC) with uv detection is reported for determination of polycyclic aromatic hydrocarbons (PAHs) in the caspian seawater. A good resolution was obtained using acetonitrile:water (40:60) as mobile-phase for separation of these compounds. The eff...

GC-FID and HPLC-DAD were developed for determination of menadione (MN) and menadione sodium bisulphite (MSB). By means of each method, quantitative analysis of MSB in commercial pharmaceutical was performed in both direct analysis of MSB and analysis of MN by converting MSB to MN with sodium carbonate. GC-FID method was carried out on the HP-5 capillary column using nitrogen gas. HPLC-DAD metho...

GC-FID and HPLC-DAD were developed for determination of menadione (MN) and menadione sodium bisulphite (MSB). By means of each method, quantitative analysis of MSB in commercial pharmaceutical was performed in both direct analysis of MSB and analysis of MN by converting MSB to MN with sodium carbonate. GC-FID method was carried out on the HP-5 capillary column using nitrogen gas. HPLC-DAD metho...