Polarity Reversed-phase chromatography and hydrophobic-interaction chromatography Centrifugation: Analytical Centrifugation. Chromatography: Protein Separation. III/Enzymes: Liquid Chromatography. Proteins: Capillary Electrophoresis; Centrifugation; Crystallization; Electrophoresis; Glycoproteins: Liquid Chromatography; High-Speed Countercurrent Chromatography; Ion Exchange; Metalloproteins: Ch...

There is no other naturally occurring defense agent against cancer that has a stronger effectthan paclitaxel, commonly known under the brand name of Taxol®. The major drawback for themore widespread use of paclitaxel and its precious precursor, 10-deacetylbaccatin III (10-DABIII), is that they require large-scale extraction from different parts of yew trees (Taxus species),cell cultures, taxane...

Centrifugal partition chromatography (CPC), pioneered by Ito in 1964, is a liquid–liquid chromatography without a sorbent, requiring two immiscible solvent phases. This is basically an outgrowth of countercurrent distribution, as developed by Craig and Post (1). In the most distinct variant of CPC, one liquid phase remains stationary while the second solvent phase passes through the stationary ...

Countercurrent chromatography (CCC) is a generic term covering all forms of liquid–liquid chromatography that use a support-free liquid stationary phase held in place by a simple centrifugal or complex centrifugal force field. Biphasic liquid systems are used with one liquid phase being the stationary phase and the other being the mobile phase. Although initiated almost 30 years ago, CCC lacked...

a blocker of postsynaptic acetylcholine receptors was isolated from venom of the eastern green mamba dendroaspis angusticeps, by gel filtration, ion-exchange and reverse phase high performance liquid chromatography. the isolated component blocks neuromuscular transmission and responses to exogenously applied acetylcholine in isolated chick biventer cervices preparations. these results suggest t...

Today, 2D online or offline liquid chromatography/mass spectrometry is state of the art for the identification of proteins from complex proteome samples in many laboratories. Both 2D liquid chromatography methods use two orthogonal liquid chromatography separation techniques. The most commonly used techniques are strong cation exchange chromatography for the first dimension and reversed phase s...

Liquid-liquid extraction or solid-phase extraction followed by gas chromatography (GC) or high-performance liquid chromatography are traditional herbicide residue determination methods for environmental samples. Solid-phase microextraction (SPME) is a solventless, fast, and sensitive alternative herbicide residue extraction method that can be applied to numerous environmental matrices. The obje...

Countercurrent chromatography (CCC) is a liquid chromatography technique in which the stationary phase is also a liquid. The solute separation is based on partitioning between the two immiscible liquid phases: the mobile phase and the support-free liquid stationary phase. Octanol-water partition coefficients (Po/w) of 17 βblockers and 17 sulphonamides were determined by CCC. Some of the Po/w co...