The protein responsible for both nucleotide pyrophosphatase (EC 3.6.1.9) and alkaline phosphodiesterase I (EC 3.1.4.1) activities was purified from MOPC 315 plasmacytoma cells. A single SDS/PAGE-purified 115-kDa protein band was used to produce a rabbit polyclonal antiserum. This antibody preparation precipitated alkaline phosphodiesterase I activity, indicating that the SDS/PAGE-purified prote...

2',3'-Cyclic-nucleotide 3'-phosphodiesterase (EC 3.1.4.37) has been widely used as a marker for myelin-oligodendrocytes in the central nervous system. Evidence has been provided that the enzyme is identical with one of the Wolfgram proteins of central nervous system myelin. The amino acid sequence of bovine 2',3'-cyclic-nucleotide 3'-phosphodiesterase was determined by both protein and cDNA seq...

A pheochromocytoma was maintained in rats from the New England Deaconess Hospital by giving the rats s.c. injections of isolated tumor cells. The animals were sac rificed 3 to 4 weeks after transplantation, the tumors were excised, and purified tumor cells were prepared. Cyclic nucleotide phosphodiesterase of the purified tumor cells was characterized and compared with that of the adrenal medul...

1. Both the Ca(2+)-pump ATPase and the polyphosphoinositide phosphodiesterase of the erythrocyte membrane can, when assayed under appropriate conditions, be activated by Ca(2+) in the micromolar range. We have therefore compared the mechanisms and affinities for Ca(2+) activation of the two enzymes in human erythrocyte membranes, to see whether the polyphosphoinositide phosphodiesterase would b...

During long-term incubation of human peripheral blood lym phocytes with phytohemagglutinin (PHA), adenosine cyclic 3': 5'-monophosphate (cyclic AMP) phosphodiesterase increases about 5-fold and guanosine cyclic 3':S'-monophosphate (cyclic GMP) phosphodiesterase increases about i 0-fold in specific activity. Under similar conditions, there is little change in either basal or fluoride-stimulated ...

In a guinea pig model of allergic asthma, we investigated the effects of the selective phosphodiesterase inhibitors rolipram (phosphodiesterase 4-selective), Org 9935 (phosphodiesterase 3-selective) and Org 20241 (dual phosphodiesterase 4/phosphodiesterase 3-selective), administered by aerosol inhalation in approximately equipotent bronchodilatory doses, on allergen-induced early and late asthm...

Agents such as prostaglandins E1 and I2 which elevate cAMP levels in platelets also increase cAMP phosphodiesterase activity. Since much of the cAMP phosphodiesterase activity in human platelets is due to the cGMP-inhibited isozyme (Macphee, C. H., Harrison, S. A., and Beavo, J. A. (1986) Proc. Natl. Acad. Sci. U. S. A. 83, 6600-6663), we examined the regulation of this isozyme by prostaglandin...

in this study, we investigated the interactive effects of intraperitoneal (i.p.) injections of three different phosphodiesterase inhibitors (pdeis) on morphine-induced analgesia in mice using tail-flick method. subcutaneous administration of morphine (1, 3 and 6 mg/kg) caused significant antinociceptive effects in a dose dependent manner. administration of pentoxifylline (12.5, 25, 50 and 100 m...

In fragments of nucleic acids bearing 3’-monophosphoryl groups, both terminals may be determined by degrading the fragment with a massive dose of a single enzyme, venom phosphodiesterase (l-5). To be reliable, this method requires a preparation of phosphodiesterase which is both highly potent and free from contaminating interfering enzymes. The most dangerous contaminant is venom endonuclease, ...