The oral toxicity of excretion products of several Photorhabdus and Xenorhabdus strains was tested on two thrips species: Frankliniella occidentalis and Thrips tabaci. Out of 46 Photorhabdus isolates and 6 Xenorhabdus isolates only 6 North American P. temperata isolates were toxic to the thrips species. After 7 days of drinking from P. temperata supernatant a mortality of 90% could be reached. ...

Bacteria communicate via small diffusible molecules to mediate group-coordinated behavior, a process designated as quorum sensing. The basic molecular quorum sensing system of Gram-negative bacteria consists of a LuxI-type autoinducer synthase producing acyl-homoserine lactones (AHLs) as signaling molecules, and a LuxR-type receptor detecting the AHLs to control expression of specific genes. Ho...

We have evaluated Photorhabdus insect-related protein (Pir) from Photorhabdus asymbiotica against dengue vectors. PirAB shows larvicidal activity against both Aedes aegypti and Aedes albopictus larvae but did not affect the Mesocyclops thermocyclopoides predator. PirAB expressed the strongest toxicity compared to PirA, PirB, or the mixture of PirA plus PirB. Whether the presence of an enterobac...

The genome of the insect pathogen Photorhabdus luminescens strain TT01 contains numerous genes predicting toxins and proteases. Within the P. luminescens TT01 genome, the products of two loci, plu 4093-plu 4092 and plu 4437-plu 4436, show oral insecticidal activity against both moth and mosquito larvae. The proteins encoded by these loci, here termed 'Photorhabdus insect related' (Pir) proteins...

Photorhabdus temperata strain M1021 is an entomopathogenic bacterium belonging to the family Enterobacteriaceae and is symbiotically associated with nematodes. The draft genome sequence of P. temperata strain M1021 consists of 5,598,253 bp with a G+C content of 43.7%, and it has 6,120 protein-coding genes.

Three Gram-stain-negative, rod-shaped, non-spore-forming bacteria, BA1 T , Q614 and PB68.1 isolated from the digestive system of Heterorhabditis entomopathogenic nematodes, were biochemically molecularly characterized to clarify their taxonomic affiliations. The 16S rRNA gene sequences these strains suggest that they belong Gammaproteobacteria, family Morganellacea genus Photorhabdus . Deeper a...

Three Gram-stain-negative, rod-shaped, non-spore-forming bacteria, BA1 T , Q614 and PB68.1 isolated from the digestive system of Heterorhabditis entomopathogenic nematodes, were biochemically molecularly characterized to clarify their taxonomic affiliations. The 16S rRNA gene sequences these strains suggest that they belong Gammaproteobacteria, family Morganellacea genus Photorhabdus . Deeper a...

Photorhabdus luminescens is a symbiont of entomopathogenic nematodes. Analysis of the genome sequence of this organism revealed a homologue of PhoP-PhoQ, a two-component system associated with virulence in intracellular bacterial pathogens. This organism was shown to respond to the availability of environmental magnesium. A mutant with a knockout mutation in the regulatory component of this sys...

xenorhabdus and photorhabdus are members of the family enterobacteriaceae, and are engaged in a mutualistic association with the entomopathogenic nematodes, steinernema and heterorhabditis, respectively. in the present study, the organic fraction of ethyl acetate bacterial crude extracts of the symbiotic bacteria, xenorhabdus stockiae and photorhabdus luminescens isolated from entomopathogenic ...

The bacterial symbiont AM7(T), isolated from a novel entomopathogenic nematode species of the genus Heterorhabditis, displays the main phenotypic traits of the genus Photorhabdus and is highly pathogenic to Galleria mellonella. Phylogenetic analysis based on a multigene approach (16S rRNA, recA, gyrB, dnaN, gltX and infB) confirmed the classification of isolate AM7(T) within the species Photorh...