Phytase, an enzyme that degrades the phosphorus storage compound phytate, has the potential to enhance phosphorus availability in animal diets when engineered into soybean (Glycine max) seeds. The phytase gene from Aspergillus niger was inserted into soybean transformation plasmids under control of constitutive and seed-specific promoters, with and without a plant signal sequence. Suspension cu...

Two experiments were conducted to study the effect of phytase supplementation to lowphosphorus starter and finisher broiler diets on mineral utilization and growth performance. All diets in this study were isonitrogenous, isocaloric and corn-soybean diets. In Experiment 1, four replicate groups of 15 commercial broiler chicks, one-day old, were randomly assigned to each of two dietary treatment...

microbial phytases were applied mainly to animal and human foodstuffs in order to improvemineral bioavailability and food processing. in addition, phytases have potentialbiotechnological application in various other fields, such as environmental protection,aquaculture and agriculture. bacillus sp. dm12, an isolate from a hot spring, produces phytase,which catalyzes the hydrolysis of phytic acid...

A bacterial isolate, Alcaligenes sp. secreting phytase (EC 3.1.3.8), was isolated and characterized. The optimum conditions for the production of phytase included a fermentation period of 96 h, pH 8.0, and the addition of 1% (w/v) maltose and 1% (w/v) beef extract to the culture medium. This enzyme was purified to homogeneity and had an apparent molecular mass of 41 kDa. The optimum pH range an...

Phytic acid is an antinutritional compound that chelates P and essential divalent cations such as Fe, Ca, Zn in cereals major staple crops wheat, maize, rice, sorghum. As a result, these cannot be absorbed by monogastric animals or humans: phytic has inhibitory effect on nutrient uptake its levels are negatively correlated with protein starch digestibility. However, can degraded the action of e...

Phytase improves the bioavailability of phytate phosphorus in plant foods to humans and animals and reduces phosphorus pollution of animal waste. Our objectives were to express an Aspergillus niger phytase gene (phyA) in Saccharomyces cerevisiae and to determine the effects of glycosylation on the phytase's activity and thermostability. A 1.4-kb DNA fragment containing the coding region of the ...

this experiment was conducted to investigate the effect of phytase superdoses alone or in combination with citric acid (ca) in canola meal-based diets severely limited in available phosphorus (pa) on growth performance, plasma phosphorus (p), and tibia ash (ta) in broilers from 22 to 42 d of age. two hundreds and eighty 21-d-old male broilers were used in 28 pens of 10 birds per each. the exper...

The use of probiotics engineered to express specific enzymes has been the subject of considerable attention in poultry industry because of increased nutrient availability and reduced cost of enzyme supplementation. Phytase enzyme is commonly added to poultry feed to improve digestibility and availability of phosphorus from plant sources. To construct a probiotic with potential of phytate degrad...

This review focuses on phytase functionality in the digestive tract of farmed non-ruminant animals and the factors influencing in vivo phytase enzyme activity. In pigs, feed phytase is mainly active in the stomach and upper part of the small intestine, and added phytase activity is not recovered in the ileum. In poultry, feed phytase activities are mainly found in the upper part of the digestiv...

the present study was carried out to investigate the effects of different levels of canola meal eithr with or without phytase addition on laying hen's performance. a number of 288 layer hens (the strains of hy-line w36) at the 60th week of age, selected as based on the same egg production percentage and body weight were, assigned to a factorial arrangement with a completely randomized desi...