In this study, genistein, a selective protein tyrosine kinase (PTK) inhibitor, inhibited peripheral blood mononuclear cell (PBMC) proliferation and interleukin-2 production from cultures that were stimulated with phytohemagglutinin (PHA), phorbol 12-myristate 13-acetate (PMA) plus A23187, or PHA plus PMA, and genistein effectively blocked the PHA plus IL-2-induced PBMC proliferation. Further, w...

Purified streptococcal mitogens (SMs) including erythrogenic exotoxin were compared with phytohemagglutinin (PHA) for their ability to sustain lymphadenopathy-associated virus (LAV) replication after the stimulation of normal human peripheral blood mononuclear cells and purified CD4+ and CD8+ T cells infected with LAV. Both SM and PHA supported LAV production in peripheral blood mononuclear and...

The bisecting N-acetylglucosamine residue is formed by UDP-N-acetylglucosamine:beta-D-mannoside-beta-1, 4-N-acetylglucosaminyltransferase III (GnT-III), a key branching enzyme for N-glycans. We found that forskolin, an adenylyl cyclase activator, markedly enhanced GnT-III at the transcriptional level in various hepatoma cells and hepatocytes, resulting in an increase of bisecting GlcNAc residue...

APA is a multigene locus that comprises the protein genes of arcelin (Arc), phytohemagglutinin (PHA) or lectin, and an ?-amylase inhibitor (?-AI). These play essential roles in defense responses legume seeds to protect against seed weevils common bean (P. vulgaris). During evolution this complex locus, PHA proteins appeared first, followed by ?-AI Arc proteins. This study compared analyzed sequ...

The mitogen reactivity of peripheral blood and bone marrow cells from 5 patients with T-cell chronic (T-CLL) and 9 patients with T-ceII acute (T-ALL) Iymphoproliferative disease was investigated. Proliferative responsiveness to phytohemagglutinin (PHA) generally was found to be reduced or completely absent. Stimulation with PHA in the presence of the tumor promoter 1 2-0-tetradecanoyl phorbol-1...

The kinetics of ribosomal RNA transcription and processing were assessed in chronic lymphocytic leukemia (CLL) lymphocytes during the initial phases of their delayed response to phytohemagglutinin. When compared to cultures of normal lymphocytes, CLL cultures developed normal augmentations in 45S rRNA precursor transcription and cleavage after a 1 hr incubation with PHA. However, failure to con...

Peripheral blood lymphocytes from normal donors and patients with chronic lymphocytic leukemia, B-cell type, were purified into T, helper T, and suppressor T lymphocytes by fluorescence-activated cell sorting using OKT3, OKT4, and OKT8 monoclonal antibodies. The maximum response of the purified subpopulations to stimulation by phytohemagglutinin (PHA) was determined by measuring the production ...

Single or repetitive i.p. injections of Phytohemagglutinin (PHA) induced a selective marked decrease in blood and bone marrow lymphocytes in mice, suggesting that PHA might have a specific immunosuppressive effect. To test this hypothesis, parallel' experiments were conducted using female C57Bl/6J (H-2b) mice as recipients and either female C3H/HeJ (H-2k) or female (C57Blx C3He) Fl (H-2b,k) hyb...

We have previously demonstrated that lentil phytohemagglutinin (lentil-PHA) binds to human platelet membranes without causing either aggregation or the release reaction. When platelets are treated with thrombin, there is an increase in lentil-PHA binding suggesting the appearance of new receptor sites on the cell surface. We prepared a lentil-PHA-ferritin conjugate using affinity chromatography...