In seed technology, the use of biocompatible materials, such as chitosan, has been demonstrated to improve germination process and establishment seedlings. This research is focused on effect a chitosan coating development sesame bean plantlets. The seeds were treated with different techniques combinations chitosan: solutions at 0.1, 0.5 1% used in film coating, flakes particle sizes 1.19 mm 0.7...

With the spread of pollinosis caused by sugi (Japanese cedar, Cryptomeria japonica) pollen, use pollen-free somatic seedlings is expected in Japan. However, there a lack knowledge on relationship between abilities during embryogenesis, initial vitro growth traits, and subsequent seedlings. In present study, we provide first basic information embryo maturation efficiency, germination, plantlet c...

In vitro seedlings were used as explants for protocorm like bodies (PLBs) production which in turn were used for regeneration purpose. PLBs were induced from the base of seedlings (1.0-1.5 cm in size) in MS + BAP (8.88 microM). After 90 days of inoculation, PLBs production rate started declining and most of the PLBs turned into plantlets. Preculture of seedlings in 1.0 microM thidiazuron (TDZ) ...

Alfalfa (Medicago sativa) plantlets were exposed to Cd or Hg to study the kinetics of diverse stress indexes. In the so-called beaker-size hydroponic system, plantlets were grown in 30 microM of Cd or Hg for 7 d. Oxidative stress took place and increased over time, a linear response being observed with Cd but not with Hg. To improve the sensitivity of the stress assays used, a micro-assay syste...

The midto long-term preservation of papaya (Carica papaya L.) would allow for the safeguarding of important germplasm. In this study, soft friable callus (SFC) and hard callus (HC) were induced from the first two true leaves of 10-day-old seedlings containing a midrib derived from the germinated seed of two cultivars (‘Rainbow’ and ‘Sunrise Solo’). Following germination on a Murashige and Skoog...

Photosynthesis of plantlets in tissue culture containers is not considered important, compared to photosynthesis ex vitro plants, due the exogenous source carbohydrates present media. However, CO2 starvation can generate a burst reactive oxygen species (ROS). We examined this phenomenon culture, since levels may become very low during light period. The research was carried out with lily scales,...

salvia leriifolia (lamiaceae) is endemic of khorasan and semnan province and an endangered plant. poor seed germination of this plant has a serious problem with the high production. the first step to improve this precious plant is to produce sterile plantlets in order to prepare explants of appropriate vigor. in this study,in vitro culture of salvia leriifolia zygotic embryo was performed throu...