نتایج جستجو برای: plasmids
تعداد نتایج: 15167 فیلتر نتایج به سال:
Cell-free protein synthesizing extracts incubated with purified preparations of colicin E(3) showed a marked decrease of their protein synthesizing activity. Incubation of ribosomes prepared from S-30 extracts with E(3) resulted in their inactivation. Their 16S RNA was found to lose a terminal fragment, similar in size to the fragment released upon in vivo treatment with E(3). Extracts derived ...
IncK plasmids are some of the main carriers of blaCTX-M-14 and blaCMY-2 genes and show high similarity to other plasmids belonging to the I complex, including IncB/O plasmids. Here, we studied the phylogenetic relationship of 37 newly sequenced IncK and IncB/O plasmids. We show that IncK plasmids can be divided into two compatible lineages named IncK1 and IncK2.
Many multidrug-resistant bacterial pathogens harbor large plasmids that encode proteins conferring resistance to antibiotics. Although the acquisition of these plasmids often enables bacteria to survive in the presence of antibiotics, it is possible that plasmids also represent a vulnerability that can be exploited in tailored antibacterial therapy. This review highlights three recently describ...
BACKGROUND The conjugative plasmid, pLS20, isolated from Bacillus subtilis natto, has an outstanding capacity for rapid self-transfer. In addition, it can function as a helper plasmid, mediating the mobilization of an independently replicating co-resident plasmid. RESULTS In this study, the oriT sequence of pLS20cat (oriTLS20) was eliminated to obtain the plasmid, pLS20catΔoriT. This resulted...
Fosfomycin resistance in Escherichia coli is rare in the United States. An extended-spectrum β-lactamase-producing E. coli clinical strain identified in Pennsylvania, USA, showed high-level fosfomycin resistance caused by the fosA3 gene. The IncFII plasmid carrying this gene had a structure similar to those found in China, where fosfomycin resistance is commonly described.
Received: 21 April, 2009 Accepted: 8 July, 2009 Abstract Background & Aims: Dimethoate as an organophosphorus compound is commonly used for crop production which is neurotoxic in human. Pseudomonas family harbor organophosphate degrading plasmids have been known as tools for cleaning environmental pollutions. Materials & Methods: Pseudomonas Putida was isolated from contaminated soil b...
Abstract Background Plasmids are mobile genetic elements, key in the dissemination of antibiotic resistance, virulence determinants and other adaptive traits bacteria. Obtaining a robust method for plasmid classification is necessary to better understand genetics epidemiology many pathogens. Until now, systems focused on specific traits, which limited their precision universality. The definitio...
Bacillus anthracis, the causative agent of anthrax in humans, livestock, and wildlife, exists a community with hundreds other species bacteria environment. Work on genetics these communities has shown that B. anthracis shares high percentage chromosomal genes both thuringiensis cereus, phenotypic differences among can result from extra-chromosomal DNA form plasmids. We developed simple hypothet...
background: tuberculosis is a disease with high morbidity, caused mainly by mycobaterium tuberculosis (m.tb.). dna vaccines show a promising future due to their unique advantages over conventional methods. the early-secreted antigen target (esat)-6 and culture filtrate protein (cfp)-10 of m.tb. antigens have been identified as vaccine candidates against mycobacteria and used as subunit vaccines...
introduction: human t cell lymphotropic l.irus type i is responsible for adult t cell leukemia/lymphoma and htlv-i associated myelopathy. the aim of this study was constructing two reporter plasmids to enable us to evaluate the effects of htlv-i tax protein upon intra cellular signalling pathways which recruit creb and nfkb proteins. material and methods: a complete coding region of bacterial b...
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