We studied the intrahepatic disposition characteristics of galactosylated polyethylenimine (Gal-PEI)/plasmid DNA (pDNA) complexes using rat liver perfusion experiment. After intraportal administration, transfection activity in liver of Gal-PEI complexes was approximately 26-fold higher than that of native PEI complexes. To evaluate the relationship between hepatic gene expression and dispositio...

In this study, combinations of bisphenol A diglycidyl ether (BPADGE) and polyethylenimine (PEI) were investigated as effective crosslinking agents for soy protein isolate (SPI) at room temperature. Three-ply plywood panels prepared with the SPI-BPADGE-PEI adhesives via a cold press process evaluated their water resistance shear strengths in accordance requirements American National Standard Har...

objective polyethylenimine (pei), a readily available synthetic polycation which has high transfection efficiency owing to its buffering capacity was introduced for transfection a few years ago. but it has been reported that pei is cytotoxic in many cell lines. in this study, in order to enhance the transfection efficiency of 10 kda pei and reduce its toxicity, hydrophobic residues were grafted...

Purpose: As human papillomavirus (HPV) is primarily responsible for the development of cervical cancer, significant efforts have been devoted to develop novel strategies for detecting and identifying HPV DNA in urine. The analysis of target DNA sequences in urine offers a potential alternative to conventional methods as a non-invasive clinical screening and diagnostic assessment tool for the de...

Self-assembly supramolecular nanoparticles (SNPs) are synthesized using polyethylenimine (PEI) crosslinked by cyclodextrins (CyDs), adamantyl doxorubicin and plasmid DNA. SNPs successfully conduct the cooperative co-delivery of chemotherapeutic anticancer drug and plasmid DNA in vitro and in vivo.

A novel co-polymer based on 2-hydroxypropyl-alpha-cyclodextrin cross-linked by low molecular weight polyethylenimine was synthesized as a gene delivery vector. The copolymer could bind and condense DNA tightly. It showed lower cytotoxicity than PEI 25kDa in SK-BR-3 cells. Transfection efficiency was increased over 5.5-fold higher than PEI 25 kDa in SK-BR-3 cells in complete serum medium. It is ...

Anatase (TiO₂) and multiwalled carbon nanotubes bearing polyethylenimine (PEI) anchored on their surface were hybridized in different proportions according to a sol-gel method. The resulting nanocomposites (TiO₂@PEI-MWCNTs), characterized by BET, XRD, XPS, SEM, and UV techniques, were found efficient catalysts for CO₂ photoreduction into formic and acetic acids in water suspension and under vis...

Polysulfonated macromolecules are known to bind selectins, adhesion membrane proteins which are broadly implicated in inflammation. Commercially available branched polyethylenimine (PEI) was reacted with chlorosulfonic acid to generate sulfonated PEI with varying degrees of sulfonation. Remaining unreacted amine groups were then used for straightforward conjugation with pyropheophoribide-a, a n...

BACKGROUND RNA interference has promising therapeutic potential. However, safe and efficacious delivery systems are necessary for its application in the clinic. MATERIALS AND METHODS An oleic acid (OA) derivative of branched polyethylenimine (PEI, M.W. 2000 Da), PEI-OA, was synthesized and evaluated for small interfering RNA (siRNA) delivery in SK-HEP-1 liver cancer cells stably transfected w...

Reversing pathologic alterations in vascular microRNA (miRNA) expression represents a potential therapeutic strategy for pulmonary hypertension. While polyethylenimine (PEI) has previously been shown to be an effective vehicle for vascular lung-directed delivery of plasmid DNA, it remains unclear whether this utility is generalizable to miRNAs. Here we show that despite elevated lung levels, th...