In lantibiotic lacticin 481 biosynthesis, LctT cleaves the precursor peptide and exports mature lantibiotic. Matrix-assisted laser desorption ionization-time of flight mass spectrometry revealed that a truncated form of lacticin 481 is produced in the absence of LctT or after cleavage site inactivation. Production of truncated lacticin 481 is 4-fold less efficient, and its specific activity is ...

Lantibiotics are ribosomally synthesized and post-translationally modified peptide natural products that contain the thioether structures lanthionine and methyllanthionine and exert potent antimicrobial activity against Gram-positive bacteria. At present, detailed modes-of-action are only known for a small subset of family members. Lacticin 481, a tricyclic lantibiotic, contains a lipid II bind...

5.1. Detection Formats 475 5.2. Food Quality and Safety Analysis 477 5.2.1. Pathogens 477 5.2.2. Toxins 479 5.2.3. Veterinary Drugs 479 5.2.4. Vitamins 480 5.2.5. Hormones 480 5.2.6. Diagnostic Antibodies 480 5.2.7. Allergens 481 5.2.8. Proteins 481 5.2.9. Chemical Contaminants 481 5.3. Medical Diagnostics 481 5.3.1. Cancer Markers 481 5.3.2. Antibodies against Viral Pathogens 482 5.3.3. Drugs ...

180 © 2011 Johnson Matthey doi:10.1595/147106711X581350 http://www.platinummetalsreview.com/ Edited by Pierluigi Barbaro and Francesca Liguori (Istituto di Chimica dei Composti Organo Metallici, Firenze, Italy), Springer, Dordrecht, The Netherlands, 2010, 459 pages, ISBN: 978-90-481-3695-7, £126.00, €139.95, US$189.00 (Print version); e-ISBN: 978-90-481-3694-4, doi:10.1007/978-90-481-3696-4 (On...

Two papers in the Journal this month examine patterns of prescribing practice. With established clinical guidelines for the management of individuals with treatment-resistant schizophrenia in mind, Howes et al (pp. 481–485) reviewed prescribing practices prior to commencement of clozapine for a sample of patients managed within one mental health service. The authors found evidence of a signific...

N. Ulman, S. G. Boxer, Science 1997, 275, 651. c) W. Knoll, Annu. Rev. Phys. Chem. 1998, 49, 569. [3] Crystallization of Nucleic Acids and Proteins: A Practical Approach (Eds: A. Ducruix, R. Giege), Oxford University Press, Oxford 1992. [4] S. D. Durbin, G. Feher, Annu. Rev. Phys. Chem. 1996, 47, 171. [5] O. D. Velev, E. W. Kaler, A. M. Lenhoff, unpublished. [6] See for example: a) D. E. Koppel...

ACKNOWLEDGEMENTS I would like to express my sincere gratitude to my advisor, Professor Xiaoming Huo for his great help and support during my graduate studies. His patience and insight make it a pleasure to work with him. Thanks are also due to other members of am also very grateful to my parents, Jiqiu Chen and Wenhui Liu, for their love and support over these four and a half years. Finally, I ...

10 Gershenson, S. I., J. Genet., 28, 297-313 (1933). 11TheYsX . YL-chromosome does not permit a decision as to whether YS and yL can conjoin intrachromosomally, even though this chromosome regularly conjoins with itself in Y8X.YL/O spermatocytes (Fig. 3i). Here "Ys" denotes with certainty only the Y8-fertility genes, for the "Ys" element itself (Fig. 3h) is morphologically unlike the short arm ...

Human immunoglobulin M (IgM) rheumatoid factors (RFs) show primary direct enzyme-linked immunosorbent assay (ELISA) reactivity with Fab rather than Fc fragments of monoclonal antibody (mAb) II-481 directed against the Fc3'-binding site of herpes simplex virus glycoprotein gE. This preferential anti-Fab specificity suggests that RFs react with antigen-binding portions of mAb II-481 as anti-idiot...