The rates of gain, catabolism, synthesis and reutilization of myofibrillar protein were measured in adult rats fed a protein-free diet, low protein diet (2% lactalbumin) or control diet (10% lactalbumin) for 14 to 31 days. Two forms of synthesis were measured: exogenous synthesis (nitrogen derived from diet) and endogenous synthesis (nitrogen derived from catabolized body protein). The rate of ...

Investigations have demonstrated a strong and positive association between dietary intact phospholipid (PL) inclusion aquatic larval growth, nevertheless, the precise molecular mechanism underlying PL on growth performance has not been well elucidated. This study aimed to investigate effects of soybean lecithin (SL) performance, liver metabolism, resistance hypoxia stress, potential mechanisms ...

Cisplatin-induced nephrotoxicity limits its use in many cancer patients. The expression of enzymes involved in polyamine catabolism, spermidine/spermine N1-acetyltransferase (SSAT) and spermine oxidase (SMOX) increase in the kidneys of mice treated with cisplatin. We hypothesized that enhanced polyamine catabolism contributes to tissue damage in cisplatin acute kidney injury (AKI). Using gene k...

Protein and amino acid metabolism is abnormal in end-stage renal disease (ESRD). Protein turnover is influenced by transmembrane amino acid transport. The effect of ESRD and hemodialysis (HD) on intracellular amino acid transport kinetics is unknown. We studied intracellular amino acid transport kinetics and protein turnover by use of stable isotopes of phenylalanine, leucine, lysine, alanine, ...

Alanine and glutamine constitute the two most important nitrogen carriers released from the muscle. We studied the intracellular amino acid transport kinetics and protein turnover in nine end-stage renal disease (ESRD) patients and eight controls by use of stable isotopes of phenylalanine, alanine, and glutamine. The amino acid transport kinetics and protein turnover were calculated with a thre...

The relentless catabolism of body protein during chronic disease may kill. Early investigators presumed that the origins of cachexia lay in the underlying neoplasia or organism usurping the host's energy stores. More recent evidence indicates that immunological and neuroendocrinological mediators produced in response to invasion amplify the catabolism of lean body tissue, diverting substrate fr...

Conjugation of recombinant human deoxyribonuclease I (rhDNase) to polyethylene glycol (PEG) 20 40 kDa was previously shown prolong the residence time rhDNase in lungs mice after pulmonary delivery while preserving its full enzymatic activity. This work aimed study fate native and PEGylated elucidate their biodistribution elimination pathways intratracheal instillation mice. In vivo fluorescence...

Abstract Global human activities, such as greenhouse emissions and pollution, are promoting global warming, environmental changes biodiversity reduction. Pristine environments those of Antarctica not immune to these phenomena, is noticeable from the increasing pace temperature shift registered within continent in recent decades. In this study, we describe first de novo transcriptome analysis en...

Background: Perioperative exacerbation of hyperglycemia and insulin resistance is associated with increased complications in patients with diabetes mellitus. We recently reported that glucose load during anesthesia with sevoflurane suppressed lipid catabolism in diabetic patients. In contrast to inhaled anesthetics, propofol solution contains triglycerides, which can be an energy source during ...