Histone deposition proteins CAF-1 and Asf-1 impact multiple aspects of chromosome formation and are essential in yeast to prevent DNA damage and avoid chromosomal rearrangement. Using fractionated yeast cell extracts, this MQP attempted to identify stimulatory factors which are necessary and sufficient for histone deposition by CAF-1 and/or Asf1. Identification of these cofactors, would provide...

Many litres of fluids are found outside cells in the human body. These fluids are rich in dissolved proteins that each have a characteristic three dimensional shape, necessary for normal function, which has been attained by the correct folding of their polypeptide chain(s). The structure of these extracellular proteins can be damaged by a variety of environmental stresses (e.g. heat and oxidati...

Growth of protein and fat tissues in animals involves increases in cell numbers and cell size to genetically programmed limits but the stage of maturity, which is highly dependent on diet, sets the time scale for relative rates of development of different tissues. Complex control systems in the body influence feed intake, and how nutrients are partitioned between different tissues, Differences ...

The association of protein deposits with neurodegeneration has become a consistent finding in a large group of etiologically diverse diseases (1, 2) (Table I). The protein deposits are generally dense fibrillar structures containing a high percentage of b-pleated sheet secondary structure and can be located in various cellular compartments as well as extracellularly. Moreover, the deposits are ...

Traditional nuclear medicine ligands were designed to target cellular receptors or transporters with a binding pocket and a defined structure-activity relationship. More recently, tracers have been developed to target pathological protein aggregations, which have less well-defined structure-activity relationships. Aggregations of proteins such as tau, α-synuclein, and β-amyloid (Aβ) have been i...

I. Food intake and rates of protein, lipid and energy deposition during growth were measured for lean and congenitally obese (fatty) Zucker rats offered from 34 d of age to slaughter at 66 d of age, one of six semi-synthetic diets containing casein (C) in the following amounts (g Clkg): 40C, moC, 150C, 3wC, 500C and 700C. 2. In Expt I, groups of four male rats were offered each diet to appetite...

The hormonal control of protein deposition in animals is a highly integrated process. Consideration of this topic not only requires a study of hormonal interactions with target tissues but also the much more general topic of growth. Growth itself is a complex and integrated process and involves the formation and enlargement of many metabolic units. Obviously maximal protein deposition in an ani...

Complement plays an important role in ischemia-reperfusion injury. We recently demonstrated that reoxygenation of hypoxic human umbilical vein endothelial cells (HUVECs) activated the classical complement pathway and augmented iC3b deposition. In the present study, we investigated the potential role of oxygen-derived free radicals, NF-kappaB and new protein synthesis in this model. HUVECs subje...

Combined therapy with clarithromycin (CLTR) and N–acetyl cysteine (NAC) may be useful in diseases with impaired oxidant-antioxidant balance, fibroblast proliferation, and collagen deposition such as pulmonary fibrosis. Activated inflammatory cells which accumulate in the lower airways may release increased amounts of reactive oxygen species (ROS) when accompanied with a deficiency in glut...