Mesophyll cell protoplasts were isolated from potato (Solanum tuberosum L. cv. Russet Burbank) leaves and induced to proliferate in culture. Protoplast division was observed only among preparations isolated from plants previously conditioned under short periods of low intensity illumination. Sustained growth and development of protoplast-derived calli (p-calli) occurred when they were maintaine...

After 1 hour, exogenous deoxyribonucleic acid was degraded within a culture medium at 25 C (pH 6) containing protoplasts of Daucus carota L. var. sativa. Low temperature incubation (1 C) or the addition of 45 millimolar sodium citrate to the medium eliminated DNase activity for at least 4.5 hours. This DNase activity was not reduced at pH 7 or 9, nor by addition of 200 millimolar adenosine 5'-t...

saffron (crocus sativus l.) protoplasts were isolated from the cells of a suspension culture or calli with a solution of cellulase, pectinase and hemicellulase and embedded in ca-alginate beads. they were cultured with or without nurse cells in ms medium supplemented with 2,4-d and 6-benzylaminopurine at 25°c. after several changes of medium, cell-clusters appeared on the surface of the ca-algi...

Protoplasts were isolated from five species of Gossypium. · Protoplast yield and viability were affected by incubation conditions, osmolarity, purification procedures, and cell source. Using an optimized procedure, highly viable protoplasts were isolated from cell suspensions, callus cultures, and leaf tissue of G. hirsutum, G. arboreum, G. k/otzschianum, G. harlmessii, and G. herbaceum. Protop...

KA-prep, a culture filtrate of Bacillus circulans KA-304 grown on a cell-wall preparation of Schizophyllum commune, has an activity to form protoplasts from S. commune mycelia. alpha-1,3-Glucanase, which was isolated from an ammonium sulfate fraction of 0-30% saturation of KA-prep, gave the protoplast-forming activity to an ammonium sulfate fraction of 30-50% saturation of KA-prep, which contai...

Switchgrass (Panicum virgatum L.) is a warm-season perennial grass that has received considerable attention as a potential dedicated biofuel and bioproduct feedstock. Genetic improvement of switchgrass is needed for better cellulosic ethanol production, especially to improve cellulose-to-lignin ratios. Cell suspension cultures offer an in vitro system for mutant selection, mass propagation, gen...

The inhibitory allelopathic activity of Pueraria montana (Kudzu), and activities two putative allelochemical isoflavones, puerarin daidzein, were evaluated using the protoplast co-culture method with digital image analysis lettuce as a recipient (DIA-PP method). Cotyledon protoplasts Kudzu isolated Cellulase R10 Driselase 20 in 0.6 M mannitol solution. Optimal hormonal condition density for gro...

We have established that polypeptides whose synthesis is reduced by 2,4-dichlorophenoxyacetic acid during in vitro culture of tobacco mesophyll protoplasts are secreted into the vacuole where they constitute the bulk of labeled proteins. In addition, these proteins continue to be synthesized in protoplast-derived cultured cells and their synthesis is strictly correlated with the size of the cel...